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rabbit anti-BAFF (CT) polyclonal antibody 4041

$445.00

Antibody summary

  • Rabbit polyclonal to BAFF (CT)
  • Suitable for: ELISA,WB,ICC,IF,IHC
  • Isotype: IgG
  • 100 µg
SKU: 4041parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

BAFF (CT)

available sizes

100 µg

rabbit anti-BAFF (CT) polyclonal antibody 4041

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 1ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentration for their application.

Positive control: Whole cell lysate from HL60 cells.
Immunogen
Peptide corresponding to aa 254-269 at the C-terminus of human BAFF.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Tumor necrosis factor ligand superfamily member 13B (B lymphocyte stimulator) (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1) (CD antigen CD257) [Cleaved into: Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis
Gene names
TNFSF13B,TNFSF13B BAFF BLYS TALL1 TNFSF20 ZTNF4 UNQ401/PRO738
Protein family
Tumor necrosis factor family
Mass
31223Da
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.; Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.; [Isoform 3]: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
Subellular location
Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted.
Tissues
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.
Structure
Homotrimer. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors.
Post-translational modification
The soluble form derives from the membrane form by proteolytic processing.; Isoform 2 is not efficiently shed from the membrane unlike isoform 1.; N-glycosylated.
Domain
TOPO_DOM 1
Target Relevance information above includes information from UniProt accession: Q9Y275
The UniProt Consortium

benchmark-antibodies_anti-baff_ct_antibody_4041_1.jpg
Western Blot Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M)
Loading: 15 µg of lysates per lane. Antibodies: BAFF 4041 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-baff_ct_antibody_4041_2.gif
Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: BAFF 4041 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-baff_ct_antibody_4041_3.gif
Western Blot Validation with Recombinant Protein
Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF 4041 (Lane 1: 0.25 µg/mL; Lane 2: 0.5 µg/mL and Lane 3: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 18kD.
benchmark-antibodies_anti-baff_ct_antibody_4041_4.jpg
Immunocytochemistry Validation of BAFF in HL60 Cells
Immunocytochemical analysis of HL60 cells using anti-BAFF antibody (4041) at 1 µg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
benchmark-antibodies_anti-baff_ct_antibody_4041_5.gif
Immunofluorescence Validation of BAFF in Human Spleen Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF with 4041 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
benchmark-antibodies_anti-baff_ct_antibody_4041_6.gif
Regulated Expression Validation of BAFF in Myeloma Patients (Tai et al., 2006)
Immunoblot analysis was performed to monitor protein expression of BAFF with anti-BAFF antibodies in multiple myeloma cells with or without BMSCs. BAFF expression in cocultures at 8hr or 24hr was up-regulated by ~3.5-fold relative to BMSCs alone.
benchmark-antibodies_anti-baff_ct_antibody_4041_7.gif
Immunohistochemistry Validation of BAFF in Thyroid of Patients with Graves Diseases (Campi et al., 2015)
BAFF expression detected by anti-BAFF antibodies (4041) was remarkably increased in thyrocytes from multinodular goiter (-) compared with either Hashimoto's thyroiditis (-) or Graves' disease (-) while no staining was found in normal thyroid tissue (-).
benchmark-antibodies_anti-baff_ct_antibody_4041_8.gif
Immunohistochemistry Validation of BAFF in Murine Cardiac Transplants at Rejection (Ye et al., 2004)
BAFF expression detected by anti-BAFF antibodies (4041) was upregulated in intragraft leukocytes due to rejection at 7 days after heart transplant.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
Western blot
IHC
ICC
Batch Information
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