| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | AU5 |
| available sizes | 100 µg |
rabbit anti-AU5 polyclonal antibody 4352
$426.00
Antibody summary
- Rabbit polyclonal to AU5
- Suitable for: WB,IP,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
rabbit anti-AU5 polyclonal antibody 4352
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:20,000 Immunocytochemistry: use at a dilution of 1:100-1:400 ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate. These ar |
| Immunogen AU5 (TDFYLK) conjugated to KLH. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| AU5 |
| Protein names AU5 epitope tag |
| Alternative names AU5 tag, AU5 epitope, TDFYLK tag |
| Function The AU5 tag is an artificial peptide epitope used for the detection and characterization of recombinant proteins using anti-AU5 antibodies. |
| Description The AU5 tag is a short peptide epitope derived from the VP1 capsid protein of bovine papillomavirus type 1 (BPV-1). It is used as a recombinant protein tag for detection, localization, and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Protein localization, Western blot, Recombinant protein |
| Sequence TDFYLK |
| Structure The AU5 tag is a 6-amino acid peptide epitope with the sequence TDFYLK derived from the VP1 protein of bovine papillomavirus type 1. |
| Function The AU5 tag is used as a molecular epitope tag for detection, localization, and characterization of recombinant proteins. |
| Detection AU5-tagged proteins can be detected using anti-AU5 antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays. |
| Applications The AU5 tag is typically fused to recombinant proteins at the N- or C-terminus to facilitate detection while minimizing effects on protein structure and function. |
| Research relevance The AU5 tag is less commonly used than FLAG, HA, Myc, or V5 tags but remains useful in recombinant protein studies and multi-tag expression systems. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-AU5 polyclonal antibody 4352 been validated for?
The rabbit anti-AU5 polyclonal antibody 4352 has been tested and is suitable for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), enzyme-linked immunosorbent assay (ELISA), and immunoprecipitation (IP).
How should the rabbit anti-AU5 polyclonal antibody 4352 be stored to maintain stability?
This antibody should be stored at 2-8°C and remains stable for at least one year under these conditions. The storage buffer is PBS at pH 7.2 containing 0.09% sodium azide (NaN3).
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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