| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| reactivity | AU1 |
| available sizes | 100 µg |
rabbit anti-AU1 polyclonal antibody 3975
$418.00
Antibody summary
- Rabbit polyclonal to AU1
- Suitable for: WB,IP,ICC,ELISA
- Isotype: IgG
- 100 µg
rabbit anti-AU1 polyclonal antibody 3975
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate These a |
| Immunogen AU1 (DTYRYI) conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS (PBS), pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| AU1 |
| Protein names AU1 epitope tag |
| Alternative names AU1 tag, AU1 epitope, DTYRYI tag |
| Function The AU1 tag is an artificial peptide epitope used for detection, localization, and characterization of recombinant proteins using anti-AU1 antibodies. |
| Description The AU1 tag is a short peptide epitope derived from the VP1 structural protein of bovine papillomavirus type 1 (BPV-1). It is used as a recombinant protein tag for detection and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Recombinant protein, Immunofluorescence, Western blot |
| Sequence DTYRYI |
| Structure The AU1 tag is a 6-amino acid peptide epitope with the sequence DTYRYI derived from the VP1 protein of bovine papillomavirus type 1. |
| Function The AU1 tag is used as a molecular epitope tag for detection and characterization of recombinant proteins. |
| Detection AU1-tagged proteins can be detected using anti-AU1 antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays. |
| Applications The AU1 tag is typically fused to recombinant proteins at the N- or C-terminus to facilitate detection without substantially affecting protein function. |
| Research relevance Although less common than FLAG, HA, Myc, or V5 tags, the AU1 tag remains useful in multi-tag experimental systems and recombinant protein studies. |
Data
 |
| Detection of AU1-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-AU1 antibody 3975 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of AU1-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-AU1 antibody 3975 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-AU1 polyclonal antibody 3975 validated for?
This antibody is validated for use in Western blotting (WB), immunoprecipitation (IP), immunocytochemistry (ICC/IF), and ELISA.
How should the rabbit anti-AU1 polyclonal antibody 3975 be stored to maintain its stability?
The antibody should be stored at 2-8°C and is stable for at least one year under these conditions.
What is the recommended dilution for using this antibody in Western blotting?
For immunoblotting, the recommended dilution range is 1:1,000 to 1:20,000.
What is the immunogen used to generate the rabbit anti-AU1 polyclonal antibody 3975?
The immunogen is the AU1 peptide sequence (DTYRYI) conjugated to keyhole limpet hemocyanin (KLH).
Which species does the host antibody come from and what is its clonality and isotype?
The antibody is a rabbit polyclonal antibody of the IgG isotype.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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