| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Apaf1 (NT) |
| available sizes | 100 µg |
rabbit anti-Apaf1 (NT) polyclonal antibody 4088
$445.00
Antibody summary
- Rabbit polyclonal to Apaf1 (NT)
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-Apaf1 (NT) polyclonal antibody 4088
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1-2ug/mL Immunohistochemistry: use at 1-2ug/mL These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Positive control: Whole cell lysate of HeLa or K562 cells. |
| Immunogen Peptide corresponding to aa 12-28 of human Apaf1. This sequence is identical in human and mouse. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Apoptotic protease-activating factor 1 (APAF-1) |
| Gene names APAF1,APAF1 KIAA0413 |
| Mass 141840Da |
| Function FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:12804598}. |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12804598}. |
| Tissues TISSUE SPECIFICITY: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. |
| Structure SUBUNIT: Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A (PubMed:25716227). {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:15262985, ECO:0000269|PubMed:21371431, ECO:0000269|PubMed:25716227}. |
| Domain DOMAIN: The CARD domain mediates interaction with APIP.; DOMAIN: The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization (By similarity). {ECO:0000250}. |
| Target Relevance information above includes information from UniProt accession: O14727 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 µg/mL. |
 |
| Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 µg/mL. |
 |
| Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 µg/mL. |
Publications
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
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