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rabbit anti-Apaf-1 (CT) polyclonal antibody 3258

$445.00

Antibody summary

  • Rabbit polyclonal to Apaf-1 (CT)
  • Suitable for: ELISA,WB,IHC-P
  • Isotype: IgG
  • 100 µg
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SKU: 3258parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Apaf1 (CT)

available sizes

100 µg

antibody
Tested applications
WB,IHC,IHC,ELISA
Recommended dilutions
Immunoblotting: use at 1-10ug/mL

Immunohistochemistry: use at 10-20ug/mL

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.

Positive control: Human heart tissue lysate.
Immunogen
Peptide corresponding to aa 1158- 1177 of human Apaf1. This sequence differs from that of mouse Apaf1 by one amino acid.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Apoptotic protease-activating factor 1 (APAF-1)
Gene names
APAF1,APAF1 KIAA0413
Mass
141840Da
Function
FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:12804598}.
Subellular location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12804598}.
Tissues
TISSUE SPECIFICITY: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Structure
SUBUNIT: Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A (PubMed:25716227). {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:15262985, ECO:0000269|PubMed:21371431, ECO:0000269|PubMed:25716227}.
Target Relevance information above includes information from UniProt accession : O14727
The UniProt Consortium

Data

benchmark-antibodies_anti-apaf1_ct_antibody_3258_1.jpg
Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 µg/mL dilution in the absence (A) or presence (B) of blocking peptide.
benchmark-antibodies_anti-apaf1_ct_antibody_3258_2.jpg
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/mL.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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