| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Apaf1 (CT) |
| available sizes | 100 µg |
rabbit anti-Apaf-1 (CT) polyclonal antibody 3258
$445.00
Antibody summary
- Rabbit polyclonal to Apaf-1 (CT)
- Suitable for: ELISA,WB,IHC-P
- Isotype: IgG
- 100 µg
rabbit anti-Apaf-1 (CT) polyclonal antibody 3258
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions Immunoblotting: use at 1-10ug/mL Immunohistochemistry: use at 10-20ug/mL These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Positive control: Human heart tissue lysate. |
| Immunogen Peptide corresponding to aa 1158- 1177 of human Apaf1. This sequence differs from that of mouse Apaf1 by one amino acid. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Apoptotic protease-activating factor 1 (APAF-1) |
| Gene names APAF1,APAF1 KIAA0413 |
| Mass 141840Da |
| Function FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:12804598}. |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12804598}. |
| Tissues TISSUE SPECIFICITY: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. |
| Structure SUBUNIT: Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A (PubMed:25716227). {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:15262985, ECO:0000269|PubMed:21371431, ECO:0000269|PubMed:25716227}. |
| Domain DOMAIN: The CARD domain mediates interaction with APIP.; DOMAIN: The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization (By similarity). {ECO:0000250}. |
| Target Relevance information above includes information from UniProt accession: O14727 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 µg/mL dilution in the absence (A) or presence (B) of blocking peptide. |
 |
| Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/mL. |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-Apaf-1 (CT) polyclonal antibody 3258 validated for?
This antibody is suitable for ELISA, Western blotting (WB), and immunohistochemistry on paraffin-embedded sections (IHC-P). Recommended concentrations are 1-10 µg/mL for immunoblotting and 10-20 µg/mL for immunohistochemistry.
How should the rabbit anti-Apaf-1 (CT) polyclonal antibody be stored to maintain stability?
The antibody should be stored at -20°C and is stable for at least one year under these conditions. It is important to avoid multiple freeze-thaw cycles to preserve antibody integrity.
What is the immunogen used to generate the rabbit anti-Apaf-1 (CT) polyclonal antibody 3258?
The immunogen is a peptide corresponding to amino acids 1158-1177 of human Apaf-1. This sequence differs from the mouse Apaf-1 by one amino acid.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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