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rabbit anti-Angiotensin Converting Enzyme 2 (ACE2) polyclonal antibody 7593

$544.00

Antibody summary

  • Rabbit polyclonal to Angiotensin Converting Enzyme 2 (ACE2)
  • Suitable for: WB
  • Isotype: Whole IgG
  • 100 µl
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Angiotensin Converting Enzyme 2 (ACE2)

available sizes

100 µL

Available product – rabbit anti-Angiotensin Converting Enzyme 2 (ACE2) polyclonal antibody 7593

antibody
Tested applications
WB
Recommended dilutions
Immunoblotting: use at dilution of 1:1,000. A band of ~92kDa is detected. Detection of ACE2 in Hela cell lysate (30ug) with #19505 diluted 1:1,000.
Immunogen
Recombinant protein corresponding to aa 406-677of human ACE2 protein.
Size and concentration
100µL and 0.73 mg/ml
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C.
Storage buffer
0.1M Tris, 0.1M Glycine, 20% glycerol, 0.01% Th
Purity
immunogen affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Angiotensin-converting enzyme 2 (EC 3.4.17.23) (Angiotensin-converting enzyme homolog) (ACEH) (Angiotensin-converting enzyme-related carboxypeptidase) (ACE-related carboxypeptidase) (EC 3.4.17.-) (Metalloprotease MPROT15) [Cleaved into: Processed angiotensin-converting enzyme 2]
Gene names
ACE2,ACE2 UNQ868/PRO1885
Protein family
Peptidase M2 family
Mass
92463Da
Function
FUNCTION: Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). {ECO:0000269|PubMed:10924499, ECO:0000269|PubMed:10969042, ECO:0000269|PubMed:11815627, ECO:0000269|PubMed:14504186, ECO:0000269|PubMed:18424768, ECO:0000269|PubMed:19021774, ECO:0000269|PubMed:19185582, ECO:0000269|PubMed:27217402}.; FUNCTION: (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. {ECO:0000269|PubMed:14647384, ECO:0000269|PubMed:15452268, ECO:0000269|PubMed:15791205, ECO:0000269|PubMed:15897467, ECO:0000269|PubMed:19901337, ECO:0000269|PubMed:24227843, ECO:0000269|PubMed:32142651, ECO:0000269|PubMed:32155444, ECO:0000269|PubMed:32221306, ECO:0000269|PubMed:32225175, ECO:0000269|PubMed:33000221, ECO:0000269|PubMed:33082294, ECO:0000269|PubMed:33432067}.; FUNCTION: [Isoform 2]: Non-functional as a carboxypeptidase. {ECO:0000269|PubMed:33077916}.; FUNCTION: [Isoform 2]: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. {ECO:0000269|PubMed:33077916, ECO:0000269|PubMed:33432184}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=angiotensin II + H2O = angiotensin-(1-7) + L-phenylalanine; Xref=Rhea:RHEA:26554, ChEBI:CHEBI:15377, ChEBI:CHEBI:58095, ChEBI:CHEBI:58506, ChEBI:CHEBI:58922; EC=3.4.17.23; Evidence={ECO:0000269|PubMed:10924499, ECO:0000269|PubMed:11815627, ECO:0000269|PubMed:19021774, ECO:0000305|PubMed:14504186}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26555; Evidence={ECO:0000269|PubMed:14504186}; CATALYTIC ACTIVITY: Reaction=angiotensin I + H2O = angiotensin-(1-9) + L-leucine; Xref=Rhea:RHEA:63532, ChEBI:CHEBI:15377, ChEBI:CHEBI:57427, ChEBI:CHEBI:147350, ChEBI:CHEBI:147351; Evidence={ECO:0000269|PubMed:10924499, ECO:0000269|PubMed:10969042, ECO:0000269|PubMed:11815627, ECO:0000269|PubMed:19021774}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63533; Evidence={ECO:0000305|PubMed:10924499, ECO:0000305|PubMed:10969042, ECO:0000305|PubMed:11815627, ECO:0000305|PubMed:19021774}; CATALYTIC ACTIVITY: Reaction=bradykinin(1-8) + H2O = bradykinin(1-7) + L-phenylalanine; Xref=Rhea:RHEA:63536, ChEBI:CHEBI:15377, ChEBI:CHEBI:58095, ChEBI:CHEBI:133069, ChEBI:CHEBI:147352; Evidence={ECO:0000269|PubMed:10969042, ECO:0000269|PubMed:11815627}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63537; Evidence={ECO:0000305|PubMed:10969042, ECO:0000305|PubMed:11815627}; CATALYTIC ACTIVITY: Reaction=H2O + neurotensin = L-leucine + neurotensin-(1-12); Xref=Rhea:RHEA:63540, ChEBI:CHEBI:15377, ChEBI:CHEBI:57427, ChEBI:CHEBI:147362, ChEBI:CHEBI:147363; Evidence={ECO:0000269|PubMed:10969042}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63541; Evidence={ECO:0000305|PubMed:10969042}; CATALYTIC ACTIVITY: Reaction=H2O + neurotensin-(1-8) = L-arginine + neurotensin-(1-7); Xref=Rhea:RHEA:63572, ChEBI:CHEBI:15377, ChEBI:CHEBI:32682, ChEBI:CHEBI:147393, ChEBI:CHEBI:147394; Evidence={ECO:0000269|PubMed:11815627}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63573; Evidence={ECO:0000305|PubMed:11815627}; CATALYTIC ACTIVITY: Reaction=H2O + kinetensin = kinetensin-(1-8) + L-leucine; Xref=Rhea:RHEA:63544, ChEBI:CHEBI:15377, ChEBI:CHEBI:57427, ChEBI:CHEBI:147364, ChEBI:CHEBI:147365; Evidence={ECO:0000269|PubMed:10969042}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63545; Evidence={ECO:0000305|PubMed:10969042}; CATALYTIC ACTIVITY: Reaction=dynorphin A-(1-13) + H2O = dynorphin A-(1-12) + L-lysine; Xref=Rhea:RHEA:63556, ChEBI:CHEBI:15377, ChEBI:CHEBI:32551, ChEBI:CHEBI:147381, ChEBI:CHEBI:147383; Evidence={ECO:0000269|PubMed:11815627}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63557; Evidence={ECO:0000305|PubMed:11815627}; CATALYTIC ACTIVITY: Reaction=apelin-13 + H2O = apelin-12 + L-phenylalanine; Xref=Rhea:RHEA:63564, ChEBI:CHEBI:15377, ChEBI:CHEBI:58095, ChEBI:CHEBI:147395, ChEBI:CHEBI:147396; Evidence={ECO:0000269|PubMed:11815627}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63565; Evidence={ECO:0000305|PubMed:11815627}; CATALYTIC ACTIVITY: Reaction=[Pyr1]apelin-13 + H2O = [Pyr1]apelin-12 + L-phenylalanine; Xref=Rhea:RHEA:63604, ChEBI:CHEBI:15377, ChEBI:CHEBI:58095, ChEBI:CHEBI:147415, ChEBI:CHEBI:147416; Evidence={ECO:0000269|PubMed:27217402, ECO:0000269|PubMed:28293165}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63605; Evidence={ECO:0000269|PubMed:27217402}; CATALYTIC ACTIVITY: Reaction=apelin-17 + H2O = apelin-16 + L-phenylalanine; Xref=Rhea:RHEA:63608, ChEBI:CHEBI:15377, ChEBI:CHEBI:58095, ChEBI:CHEBI:147421, ChEBI:CHEBI:147422; Evidence={ECO:0000269|PubMed:27217402}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63609; Evidence={ECO:0000269|PubMed:27217402}; CATALYTIC ACTIVITY: Reaction=beta-casomorphin-7 + H2O = beta-casomorphin-6 + L-isoleucine; Xref=Rhea:RHEA:63568, ChEBI:CHEBI:15377, ChEBI:CHEBI:58045, ChEBI:CHEBI:147390, ChEBI:CHEBI:147391; Evidence={ECO:0000269|PubMed:11815627}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63569; Evidence={ECO:0000305|PubMed:11815627}; CATALYTIC ACTIVITY: Reaction=H2O + neocasomorphin = L-isoleucine + neocasomorphin-(1-5); Xref=Rhea:RHEA:63600, ChEBI:CHEBI:15377, ChEBI:CHEBI:58045, ChEBI:CHEBI:147417, ChEBI:CHEBI:147418; Evidence={ECO:0000269|PubMed:11815627}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63601; Evidence={ECO:0000305|PubMed:11815627};
Subellular location
SUBCELLULAR LOCATION: [Processed angiotensin-converting enzyme 2]: Secreted {ECO:0000269|PubMed:15983030, ECO:0000269|PubMed:33713620}.; SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18424768}; Single-pass type I membrane protein {ECO:0000255}. Cytoplasm {ECO:0000250|UniProtKB:Q8R0I0}. Cell projection, cilium {ECO:0000269|PubMed:33432184}. Apical cell membrane {ECO:0000269|PubMed:33432184}. Note=Detected in both cell membrane and cytoplasm in neurons. {ECO:0000250|UniProtKB:Q8R0I0}.; SUBCELLULAR LOCATION: [Isoform 2]: Apical cell membrane {ECO:0000269|PubMed:33432184}.
Tissues
TISSUE SPECIFICITY: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level) (PubMed:15141377). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level) (PubMed:15141377). Expressed in the renal proximal tubule and the small intestine (at protein level) (PubMed:18424768). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level) (PubMed:10969042, PubMed:10924499, PubMed:15231706, PubMed:12459472, PubMed:15671045, PubMed:32715618, PubMed:32170560). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level) (PubMed:32425701, PubMed:15141377, PubMed:32715618, PubMed:32170560, PubMed:33432184). Expressed in nasal epithelial cells (at protein level) (PubMed:33432184, PubMed:32333915). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells (PubMed:32413319, PubMed:32327758, PubMed:32358202). Coexpressed with TMPRSS4 within mature enterocytes (PubMed:32404436). {ECO:0000269|PubMed:10924499, ECO:0000269|PubMed:10969042, ECO:0000269|PubMed:12459472, ECO:0000269|PubMed:15141377, ECO:0000269|PubMed:15231706, ECO:0000269|PubMed:15671045, ECO:0000269|PubMed:18424768, ECO:0000269|PubMed:32170560, ECO:0000269|PubMed:32327758, ECO:0000269|PubMed:32333915, ECO:0000269|PubMed:32358202, ECO:0000269|PubMed:32404436, ECO:0000269|PubMed:32413319, ECO:0000269|PubMed:32425701, ECO:0000269|PubMed:32715618, ECO:0000269|PubMed:33432184}.; TISSUE SPECIFICITY: [Isoform 2]: Expressed in nasal and bronchial epithelial cells (at protein level). {ECO:0000269|PubMed:33432184}.
Structure
SUBUNIT: Homodimer (PubMed:32132184). Interacts with the catalytically active form of TMPRSS2 (PubMed:21068237). Interacts with SLC6A19; this interaction is essential for expression and function of SLC6A19 in intestine (By similarity). Interacts with ITGA5:ITGB1 (PubMed:15276642, PubMed:33102950). Probably interacts (via endocytic sorting signal motif) with AP2M1; the interaction is inhibited by phosphorylation of Tyr-781 (PubMed:33436498). Interacts (via PDZ-binding motif) with NHERF1 (via PDZ domains); the interaction may enhance ACE2 membrane residence (PubMed:34189428). {ECO:0000250|UniProtKB:Q8R0I0, ECO:0000269|PubMed:15276642, ECO:0000269|PubMed:21068237, ECO:0000269|PubMed:32132184, ECO:0000269|PubMed:33102950, ECO:0000269|PubMed:33436498, ECO:0000269|PubMed:34189428}.; SUBUNIT: (Microbial infection) Interacts with SARS coronavirus/SARS-CoV spike protein. {ECO:0000269|PubMed:14647384, ECO:0000269|PubMed:15452268, ECO:0000269|PubMed:15791205, ECO:0000269|PubMed:32225175}.; SUBUNIT: (Microbial infection) Interacts with SARS coronavirus-2/SARS-CoV-2 spike protein (via RBD domain). {ECO:0000269|PubMed:32075877, ECO:0000269|PubMed:32132184, ECO:0000269|PubMed:32155444, ECO:0000269|PubMed:32225175, ECO:0000269|PubMed:32753553, ECO:0000269|PubMed:33102950, ECO:0000269|PubMed:33432067, ECO:0000269|PubMed:36228039, ECO:0000269|PubMed:36735681, ECO:0000269|PubMed:36757924}.; SUBUNIT: (Microbial infection) Interacts with human coronavirus NL63 spike protein. {ECO:0000269|PubMed:19901337}.; SUBUNIT: (Microbial infection) Interacts with human coronavirus NL63/HCoV-NL63 spike glycoprotein. {ECO:0000269|PubMed:15897467}.; SUBUNIT: [Processed angiotensin-converting enzyme 2]: (Microbial infection) Interacts with SARS coronavirus-2/SARS-CoV-2 spike protein; the interaction is increased by AVP/Arg-vasopressin with which they may form a complex. {ECO:0000269|PubMed:33713620}.
Post-translational modification
PTM: N-glycosylation on Asn-90 may limit SARS infectivity. {ECO:0000269|PubMed:10924499, ECO:0000269|PubMed:14647384, ECO:0000269|PubMed:14754895, ECO:0000269|PubMed:15084671, ECO:0000269|PubMed:19159218}.; PTM: Proteolytic cleavage by ADAM17 generates a secreted form (PubMed:15983030, PubMed:33713620). Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. {ECO:0000269|PubMed:15983030, ECO:0000269|PubMed:21068237, ECO:0000269|PubMed:21563828, ECO:0000269|PubMed:24227843, ECO:0000269|PubMed:32132184, ECO:0000269|PubMed:33713620}.; PTM: Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains. {ECO:0000305|PubMed:33436497, ECO:0000305|PubMed:33436498}.
Domain
TOPO_DOM 1
Biotechnology
BIOTECHNOLOGY: An engeneered stable, dimeric and secreted receptor with combined mutations that increase the affinity for human coronavirus SARS-CoV-2 spike protein shows potent SARS-CoV and SARS-CoV-2 neutralization in vitro. {ECO:0000269|PubMed:32753553}.
Target Relevance information above includes information from UniProt accession : Q9BYF1
The UniProt Consortium

Data

benchmark-antibodies_anti-angiotensin_converting_enzyme_2_ace2_7593_1.j
Immunoblotting: use at dilution of 1:1,000. A band of ~92kDa is detected. Detection of ACE2 in Hela cell lysate (30ug) with #7593 diluted 1:1,000.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product
Western blot
IHC
ICC

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