| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | AIF (NT) |
| available sizes | 100 µg |
rabbit anti-AIF (NT) polyclonal antibody 4440
$445.00
Antibody summary
- Rabbit polyclonal to AIF (NT)
- Suitable for: ELISA,WB,IF
- Isotype: IgG
- 100 µg
rabbit anti-AIF (NT) polyclonal antibody 4440
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1-10ug/mL. Immunocytochemistry: use at 2-5ug/mL.Immunocytochemical staining of AIF in Jurkat cells with AIF(NT) antibody at 2 ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. P |
| Immunogen Peptide corresponding to aa 109- 122 at the N-terminus of human AIF. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Connector enhancer of kinase suppressor of ras 1 (Connector enhancer of KSR 1) (CNK homolog protein 1) (CNK1) (hCNK1) (Connector enhancer of KSR-like) |
| Protein family 410 |
| Function May function as an adapter protein or regulator of Ras signaling pathways. |
| Pathway 304 |
| Subellular location Cytoplasm. Membrane ; Peripheral membrane protein . |
| Structure Interacts with RHO and RALGDS. |
| Post-translational modification Phosphorylated on tyrosine. |
| Target Relevance information above includes information from UniProt accession: O95381 |
| The UniProt Consortium |
Data
 |
| Western Blot Validation in Human Cell Lines Loading: 15 µg of lysates per lane. Antibodies: AIF 4440, (1 µg /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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| Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 µg of lysates per lane. Antibodies: AIF 2267, (1 µg/mL), AIF 4440, (1 µg/mL), AIF 2301, (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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| Immunofluorescence Validation of AIF in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling AIF with 4440 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). |
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| KD and Induced Validation of AIF in H1299 Cells(Stambolsky et al., 2006) Western blot analysis of AIF knockdown with anti-AIF antibodies in H1299 cells. AIF expression was disrupted in AIF knockdown cells (siRNA1 and siRNA4). An increased expression of AIF was induced by ZnCl2 treatment, which was not observed in AIF knockdown cells. |
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| KD Validation of AIF in AIF Silenced Stable Cells(Apostolova et al., 2006) AIF silencing is sustained in stable cell lines. Western blot analysis ofstable lines AIF-1-10, AIF-2-4 and pU6-2 using anti-AIF antibodies. AIF protein was disrupted after AIF silencing with AIF siRNA (AIF-1-10 and AIF-2-4) as compared to control (Hep3B and pU6-2). |
 |
| Immunofluorescence Validation of AIF in Rat Hippocampal Neurons (Hofer et al., 2011) (G-L) After exposure to bacterial components, AIF colocalized in mature neurons (MAP2; I, L), immature neurons (DcX;H, K), and stem/progenitor cells (Nestin; G, J). AIF expression was detected by anti-AIF antibodies. |
 |
| Subcellular Localization Validation of AIF in mononuclear cells (Gupta et al., 2424) A shows mononuclear cells (MNCs) alone, B shows MNCs transfected with control plasmid, C shows MNCs transfected with Bcl-2 expression plasmid. Overlay is of Mitotracker (red) and AIF (green). Hoechst 33258 dye is used to examine chromatin fragmentation. The release of AIF form mitochondria is detected by anti-AIF antibodies. |
 |
| Induced Expression Validation of AIF in U937 Cells (Ikai et al., 2006) Release of AIF at 48 h after the treatment with 30 uM magnolol examined by Western blotAnalysis with anti-AIF antibodies. AIF release was markedly increased 48h after magnolol treatment. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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