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rabbit anti-AIF (CT) polyclonal antibody 5029

$445.00

Antibody summary

  • Rabbit polyclonal to AIF (CT)
  • Suitable for: ELISA,WB,ICC
  • Isotype: IgG
  • 100 µg
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SKU: 5029parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

AIF (CT)

available sizes

100 µg

antibody
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 0.5-2ug/mL.

Immunocytochemistry: use at 5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: K562 cells or whole cell lysate.
Immunogen
Peptide corresponding to aa 593- 606 at the C-terminus of human AIF. This sequence is identical to those of mouse and rat AIF.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
No information
target relevance

Data

benchmark-antibodies_anti-aif_ct_antibody_5029_1.gif
Western Blot Validation in Human Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: AIF 5029, (2 µg /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-aif_ct_antibody_5029_2.gif
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: AIF 2267, (1 µg/mL), AIF 2239, (1 µg/mL), AIF 5029, (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-aif_ct_antibody_5029_3.gif
Western Blot Validation in Mouse and Rat Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: AIF 5029, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-aif_ct_antibody_5029_4.jpg
Western Blot Validation in K562 Cell Line
Loading: 15 µg of lysates per lane. Antibodies: AIF 5029, (A: 0.5 µg/mL, B: 1 µg/mL, C: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-aif_ct_antibody_5029_5.jpg
Immunocytochemistry Validation of AIF in K562 Cells
Immunocytochemical analysis of K562 cells using anti-AIF antibody (5029) at 5 µg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
benchmark-antibodies_anti-aif_ct_antibody_5029_6.gif
KD and Induced Validation of AIF in H1299 Cells(Stambolsky et al., 2006)
Western blot analysis of AIF knockdown with anti-AIF antibodies in H1299 cells. AIF expression was disrupted in AIF knockdown cells (siRNA1 and siRNA4). An increased expression of AIF was induced by ZnCl2 treatment, which was not observed in AIF knockdown cells.
benchmark-antibodies_anti-aif_ct_antibody_5029_7.gif
KD Validation of AIF in AIF Silenced Stable Cells(Apostolova et al., 2006)
AIF silencing is sustained in stable cell lines. Western blot analysis ofstable lines AIF-1-10, AIF-2-4 and pU6-2 using anti-AIF antibodies. AIF protein was disrupted after AIF silencing with AIF siRNA (AIF-1-10 and AIF-2-4) as compared to control (Hep3B and pU6-2).
benchmark-antibodies_anti-aif_ct_antibody_5029_8.gif
Immunofluorescence Validation of AIF in Rat Hippocampal Neurons (Hofer et al., 2011)
(G-L) After exposure to bacterial components, AIF colocalized in mature neurons (MAP2; I, L), immature neurons (DcX;H, K), and stem/progenitor cells (Nestin; G, J). AIF expression was detected by anti-AIF antibodies.
benchmark-antibodies_anti-aif_ct_antibody_5029_9.gif
Subcellular Localization Validation of AIF in mononuclear cells (Gupta et al., 2424)
A shows mononuclear cells (MNCs) alone, B shows MNCs transfected with control plasmid, C shows MNCs transfected with Bcl-2 expression plasmid. Overlay is of Mitotracker (red) and AIF (green). Hoechst 33258 dye is used to examine chromatin fragmentation. The release of AIF form mitochondria is detected by anti-AIF antibodies.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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