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rabbit anti-Acinus (IN) polyclonal antibody 8867

$445.00

Antibody summary

  • Rabbit polyclonal to Acinus (IN)
  • Suitable for: ELISA,WB,ICC,IF
  • Isotype: IgG
  • 100 µg
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SKU: 8867parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Acinus (IN)

available sizes

100 µg

antibody
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 1-10ug/mL

Immunocytochemistry: use at 5-10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: K562 cells or whole cell lysate.
Immunogen
Peptide corresponding to aa 775- 789 of human AcinusL, aa 17-31 of human Acinus S and aa 48-62 of human AcinusS' which differ from those of mouse Acinus by one amino acid.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Apoptotic chromatin condensation inducer in the nucleus (Acinus)
Gene names
ACIN1,ACIN1 ACINUS KIAA0670
Mass
151862Da
Function
FUNCTION: Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells. {ECO:0000269|PubMed:10490026, ECO:0000269|PubMed:12665594, ECO:0000269|PubMed:18559500, ECO:0000269|PubMed:22203037, ECO:0000269|PubMed:22388736}.
Subellular location
SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Nucleus, nucleoplasm. Note=Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.
Tissues
TISSUE SPECIFICITY: Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.
Structure
SUBUNIT: Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner. {ECO:0000269|PubMed:12665594, ECO:0000269|PubMed:16314458, ECO:0000269|PubMed:18559500, ECO:0000269|PubMed:19387494, ECO:0000269|PubMed:20966198, ECO:0000269|PubMed:22388736}.
Post-translational modification
PTM: Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1. {ECO:0000269|PubMed:18559500}.; PTM: Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.
Target Relevance information above includes information from UniProt accession : Q9UKV3
The UniProt Consortium

Data

benchmark-antibodies_anti-acinus_in_antibody_8867_1.jpg
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (IN) at 1 µg/mL.
benchmark-antibodies_anti-acinus_in_antibody_8867_2.jpg
Immunocytochemistry of Acinus in K562 cells with Acinus antibody at 5 µg/mL.
benchmark-antibodies_anti-acinus_in_antibody_8867_3.jpg
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 µg/mL.
benchmark-antibodies_anti-acinus_in_antibody_8867_4.gif
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 µg/mL. Green: Acinus Antibody (8867)
Blue: DAPI staining

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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