| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2a |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Rotavirus Group Antigen |
| available sizes | 100 µg |
mouse anti-Rotavirus A monoclonal antibody (3C10) 2823
$235.00
Antibody summary
- Mouse monoclonal to Rotavirus A
- Suitable for: WB,ELISA,IF
- Isotype: IgG2a
- 100 µg
mouse anti-Rotavirus A monoclonal antibody (3C10) 2823
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions ELISA, Immunoblotting, and IHC Immunofluorescence Detection of RV strain SA11 in paraformaldehyde-fixed HT29 cells 9 hours post-infection. Cells were permeabilized with 0.1% Triton-X100 for 15 minutes at 4°C prior to staining. Endusers should determine optimal concentrations for their a |
| Immunogen Purified bovine rotavirus cell culture adapted strain Mr |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 4°C or -20°C. If frozen, store in appropriate aliquots to avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4, 0.09% NaN3. |
| Purity protein affinity purification |
| Clonality monoclonal |
| Isotype IgG2a |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2a - Isotype Control |
| target relevance |
|---|
| Protein names Rotavirus |
| Structure Rotaviruses are the most common cause of diarrhoeal disease among infants and young children.Nearly every child in the world is infected with a rotavirus at least once by the age of five. Immunity develops with each infection, so subsequent infections are less severe. Adults are rarely affected. Rotavirus is a genus of double-stranded RNA viruses in the family Reoviridae.There are nine species of the genus, referred to as A, B, C, D, F, G, H, I and J. Rotavirus A, the most common species, causes more than 90% of rotavirus infections in humans. |
Data
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| Detection of RV strain SA11 in paraformaldehyde-fixed HT29 cells 9 hours post-infection. Cells were permeabilized with 0.1% Triton-X100 for 15 minutes at 4°C prior to staining. |
FAQ & Publications
Frequently Asked Questions
What applications is the mouse anti-Rotavirus A monoclonal antibody (3C10) validated for?
This antibody has been tested and is suitable for Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence (IF) applications including immunocytochemistry (ICC).
How should the mouse anti-Rotavirus A monoclonal antibody be stored to maintain stability?
The antibody is stable for at least one year when stored at 4°C or -20°C. If frozen, it should be aliquoted appropriately to avoid repeated freeze-thaw cycles.
What is the host species and isotype of the anti-Rotavirus A monoclonal antibody (3C10)?
The antibody is a mouse monoclonal antibody of the IgG2a isotype.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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