mouse anti-RFP monoclonal antibody (RF5R) 1018

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to RFP
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG1
100 µg

available sizes

Clear

SKU: 1018parent Categories: antibody, epitope tag Tag: RFP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-RFP monoclonal antibody (RF5R) 1018

antibody
Database link: Q9U6Y8
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen RFP from the?Discosoma sp.?(sea anemone) N-terminal peptide-KLH conjugates
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance

Protein expression of RFP and RFP tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, RFP fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and RFP

Biotechnology
Red fluorescent proteins (RFPs) have become invaluable tools in scientific research, particularly in the fields of cell biology, molecular imaging, and fluorescence microscopy. RFPs are a group of genetically engineered proteins that emit red light when excited by specific wavelengths of light. Due to their spectral properties, RFPs are highly compatible with commonly used green fluorescent proteins (GFPs) and yellow fluorescent proteins (YFPs), allowing for the simultaneous visualization of multiple cellular structures or processes. Researchers utilize RFPs as versatile molecular tags to track and study specific proteins, organelles, or cellular processes in living cells and organisms. By fusing RFPs to proteins of interest, scientists can monitor their subcellular localization, dynamics, and interactions in real-time, providing crucial insights into cellular mechanisms and protein function. RFPs have also proven valuable in tracking cell migration, tissue development, and gene expression in vivo. The versatility, brightness, and photostability of red fluorescent proteins have revolutionized fluorescence-based imaging techniques, enabling a deeper understanding of complex biological processes and advancing our knowledge across various research disciplines.

Data

1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with TurboRFP (2), and transfected with dsRed (3).

Publications

pmid	title	authors	citation
26071555	Visualization of Fra-1/AP-1 activation during LPS-induced inflammatory lung injury using fluorescence optical imaging.	Subbiah Rajasekaran, Chandramohan R Tamatam, Haranatha R Potteti, Venu Raman, Jae-Woo Lee, Michael A Matthay, Dolly Mehta, Narsa M Reddy, Sekhar P Reddy	Am J Physiol Lung Cell Mol Physiol 309:L414-24

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.