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mouse anti-RbAp48 monoclonal antibody (15G12) 2844

$503.00

Antibody summary

  • Mouse monoclonal to RbAp48
  • Suitable for: WB,ICC/IF,IHC-P,IP,EMSA
  • Isotype: IgG2a
  • 100 µg
SKU: 2844parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG2a

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

RbAp48

available sizes

100 µg

Available product – mouse anti-RbAp48 monoclonal antibody (15G12) 2844

antibody
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
Immunoblotting: use at 1-5ug/mL. A band of ~48kDa is detected. Detection of RbAp48 in K562, THP-1, and HL-60 whole cell extracts (30ug), separated by 10% SDS-PAGE, with #3111 at 2ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their
Immunogen
GST fusion protein corresponding to aa 1-425 of human RbAp48.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable at -20°C for at least one (1) year; store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG2a
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG2a - Isotype Control
target relevance
Protein names
Retinoblastoma-associated protein (p105-Rb) (p110-RB1) (pRb) (Rb) (pp110)
Gene names
RB1,RB1
Protein family
Retinoblastoma protein (RB) family
Mass
106159Da
Function
FUNCTION: Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {ECO:0000250|UniProtKB:P13405, ECO:0000250|UniProtKB:P33568, ECO:0000269|PubMed:10499802, ECO:0000269|PubMed:15084261}.; FUNCTION: (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {ECO:0000269|PubMed:1316611, ECO:0000269|PubMed:17974914, ECO:0000269|PubMed:18701596, ECO:0000269|PubMed:2839300, ECO:0000269|PubMed:8892909}.
Subellular location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20940255}. Note=During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. {ECO:0000269|PubMed:20940255}.
Tissues
TISSUE SPECIFICITY: Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) (PubMed:20940255). {ECO:0000269|PubMed:20940255}.
Structure
SUBUNIT: Interacts with ATAD5. Interacts with PRMT2, CDK1 and CDK2 (By similarity). The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor (PubMed:8336704, PubMed:20940255). Interacts with heterodimeric E2F/DP transcription factor complexes containing TFDP1 and either E2F1, E2F3, E2F4 or E2F5, or TFDP2 and E2F4. The unphosphorylated form interacts with EID1, ARID3B, KDM5A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with SNW1, AATF, DNMT1, LIN9, LMNA, KMT5B, KMT5C, PELP1, UHRF2 and TMPO-alpha. May interact with NDC80. Interacts with GRIP1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1 and LIMD1. Interacts with SMARCA4/BRG1 and HDAC1 (By similarity). Interacts with PSMA3 and USP4. Interacts (when methylated at Lys-860) with L3MBTL1. Interacts with CHEK2; phosphorylates RB1. Interacts with CEBPA (PubMed:15107404). P-TEFB complex interacts with RB1; promotes phosphorylation of RB1 (PubMed:12037672). Interacts with RBBP9; the interaction disrupts RB1 binding to E2F1 (By similarity). Interacts with KAT2B/PCAF and EP300/P300 (By similarity). Interacts with PAX5 (PubMed:10197586). {ECO:0000250|UniProtKB:P13405, ECO:0000250|UniProtKB:P33568, ECO:0000269|PubMed:10197586, ECO:0000269|PubMed:10409732, ECO:0000269|PubMed:10446990, ECO:0000269|PubMed:10779342, ECO:0000269|PubMed:10869426, ECO:0000269|PubMed:10888886, ECO:0000269|PubMed:11223246, ECO:0000269|PubMed:11484059, ECO:0000269|PubMed:11571652, ECO:0000269|PubMed:12037672, ECO:0000269|PubMed:12450794, ECO:0000269|PubMed:12466551, ECO:0000269|PubMed:12475961, ECO:0000269|PubMed:12682072, ECO:0000269|PubMed:12695505, ECO:0000269|PubMed:15107404, ECO:0000269|PubMed:15538385, ECO:0000269|PubMed:15542589, ECO:0000269|PubMed:15803180, ECO:0000269|PubMed:15927959, ECO:0000269|PubMed:15949438, ECO:0000269|PubMed:16214886, ECO:0000269|PubMed:16337594, ECO:0000269|PubMed:16360038, ECO:0000269|PubMed:16645588, ECO:0000269|PubMed:17380128, ECO:0000269|PubMed:17996702, ECO:0000269|PubMed:20870719, ECO:0000269|PubMed:20940255, ECO:0000269|PubMed:21952639, ECO:0000269|PubMed:7525595, ECO:0000269|PubMed:7935440, ECO:0000269|PubMed:8336704, ECO:0000269|PubMed:8414517, ECO:0000269|PubMed:9858607}.; SUBUNIT: (Microbial infection) Interacts with adenovirus E1A protein. {ECO:0000269|PubMed:17974914}.; SUBUNIT: (Microbial infection) Interacts with HPV E7 protein. {ECO:0000269|PubMed:1316611}.; SUBUNIT: (Microbial infection) Interacts with SV40 large T antigen. {ECO:0000269|PubMed:1316611, ECO:0000269|PubMed:2839300}.; SUBUNIT: (Microbial infection) Interacts with human cytomegalovirus/HHV-5 proteins UL82 and UL123. {ECO:0000269|PubMed:12626766, ECO:0000269|PubMed:8892909}.; SUBUNIT: (Microbial infection) Interacts with molluscum contagiosum virus protein MC007. {ECO:0000269|PubMed:18701596}.
Post-translational modification
PTM: Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis. {ECO:0000269|PubMed:10499802, ECO:0000269|PubMed:8114739}.; PTM: N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1. {ECO:0000250, ECO:0000269|PubMed:15084261, ECO:0000269|PubMed:15809340, ECO:0000269|PubMed:16360038, ECO:0000269|PubMed:17380128, ECO:0000269|PubMed:1756735, ECO:0000269|PubMed:20870719, ECO:0000269|PubMed:22787429}.; PTM: Acetylated during keratinocyte differentiation. Acetylation at Lys-873 and Lys-874 regulates subcellular localization. Can be deacetylated by SIRT1. {ECO:0000269|PubMed:20940255}.
Target Relevance information above includes information from UniProt accession : P06400
The UniProt Consortium

Data

benchmark-antibodies_anti-rbap48_antibody_2844_1.jpg
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RbAp48 + RbAp46(2844) antibody at 1:200 dilution.
benchmark-antibodies_anti-rbap48_antibody_2844_2.jpg
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RbAp48 + RbAp46(2844) antibody at 1:200 dilution.
benchmark-antibodies_anti-rbap48_antibody_2844_3.jpg
RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (2844) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
benchmark-antibodies_anti-rbap48_antibody_2844_4.jpg
RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (2844) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
benchmark-antibodies_anti-rbap48_antibody_2844_5.jpg
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 + RbAp46 antibody [15G12] (2844) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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Protocols

relevant to this product
Western blot
IHC
ICC

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