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mouse anti-Rad50 monoclonal antibody (2C6) 6741

$503.00

Antibody summary

  • Mouse monoclonal to Rad50
  • Suitable for: WB,ICC/IF,IHC-P,IP,IHC,in vitro
  • Isotype: IgG1
  • 100 µg
SKU: 6741parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Rad50

available sizes

100 µg

Available product – mouse anti-Rad50 monoclonal antibody (2C6) 6741

antibody
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
Immunoblotting,

Immunoprecipitation: use at 0.1-2 ug/mL.

Positive control: Raji cells.
Immunogen
GST fusion protein corresponding to the complete coding region (aa 1-425) of Rad50 expressed in E. coli.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Storage buffer
PBS, pH 7.4
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
DNA repair protein RAD50 (hRAD50) (EC 3.6.-.-)
Gene names
RAD50,RAD50
Protein family
SMC family, RAD50 subfamily
Mass
153892Da
Function
FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). {ECO:0000269|PubMed:10888888, ECO:0000269|PubMed:11741547, ECO:0000269|PubMed:15064416, ECO:0000269|PubMed:9590181, ECO:0000269|PubMed:9651580, ECO:0000269|PubMed:9705271}.
Subellular location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10783165, ECO:0000269|PubMed:15916964, ECO:0000269|PubMed:26215093}. Chromosome, telomere {ECO:0000269|PubMed:10888888}. Chromosome {ECO:0000269|PubMed:26215093}. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. {ECO:0000269|PubMed:10783165, ECO:0000269|PubMed:15916964, ECO:0000269|PubMed:26215093}.
Tissues
TISSUE SPECIFICITY: Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. {ECO:0000269|PubMed:8756642}.
Structure
SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN (PubMed:8756642, PubMed:9590181, PubMed:9705271, PubMed:10839544, PubMed:26215093). As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites (PubMed:26215093). Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN (PubMed:10783165). Found in a complex with TERF2 (PubMed:10888888). Interacts with RINT1 (PubMed:11096100). Interacts with BRCA1 via its N-terminal domain (PubMed:10426999). Interacts with DCLRE1C/Artemis (PubMed:15456891, PubMed:15723659). Interacts with MRNIP (PubMed:27568553). Interacts with CYREN (via XLF motif) (By similarity). {ECO:0000250|UniProtKB:P70388, ECO:0000269|PubMed:10426999, ECO:0000269|PubMed:10783165, ECO:0000269|PubMed:10839544, ECO:0000269|PubMed:10888888, ECO:0000269|PubMed:11096100, ECO:0000269|PubMed:15456891, ECO:0000269|PubMed:15723659, ECO:0000269|PubMed:26215093, ECO:0000269|PubMed:27568553, ECO:0000269|PubMed:8756642, ECO:0000269|PubMed:9590181, ECO:0000269|PubMed:9705271}.; SUBUNIT: (Microbial infection) Interacts with herpes simplex virus 1 protein UL12 (PubMed:20943970). {ECO:0000269|PubMed:20943970}.
Target Relevance information above includes information from UniProt accession : Q92878
The UniProt Consortium

Data

benchmark-antibodies_anti-rad50_antibody_6741_1.jpg
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (6741) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.
benchmark-antibodies_anti-rad50_antibody_6741_2.jpg
Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (6741) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
benchmark-antibodies_anti-rad50_antibody_6741_3.jpg
Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (6741) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
benchmark-antibodies_anti-rad50_antibody_6741_4.jpg
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (6741) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
benchmark-antibodies_anti-rad50_antibody_6741_5.jpg
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (6741) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
benchmark-antibodies_anti-rad50_antibody_6741_6.jpg
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (6741) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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