| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2b |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-NeuN monoclonal antibody (1B7) 2083
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to NeuN
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG2b
- 100 µg, 25 µg, 1 mg
mouse anti-NeuN monoclonal antibody (1B7) 2083
| target relevance |
|---|
| NeuN, also known as Fox-3 or RBFOX3, is a well-established neuronal marker widely used in neuroscience research. It is a nuclear protein that is selectively expressed in post-mitotic neurons, making it an essential tool for identifying and characterizing neuronal populations in various tissues and experimental models. NeuN immunohistochemistry, facilitated by specific antibodies targeting NeuN, allows researchers to visualize and quantify neuronal cell bodies, providing valuable insights into neuronal distribution, density, and morphology. This marker is particularly valuable for studying neurodevelopment, neurodegenerative diseases, and neuronal plasticity in response to different experimental conditions or treatments. Moreover, NeuN antibodies have proven instrumental in identifying and differentiating neurons from other cell types, such as glial cells, in complex neural tissues. Click for more on: cell markers and NeuN |
| Protein names RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen) |
| Gene names RBFOX3,RBFOX3 |
| Mass 33873Da |
| Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). |
| Subellular location Nucleus Cytoplasm Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment. |
| Target Relevance information above includes information from UniProt accession: A6NFN3 |
| The UniProt Consortium |
Data
 |
| Immunofluorescent analysis of rat brain stem costained with mouse mAb to FOX3/NeuN 2083 in green, and chicken pAb to microtubule associated protein 2 (MAP2) in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The FOX3/NeuN antibody selectively stains nuclei and the proximal cytoplasm of neuronal cells while the MAP2 antibody labels dendrites and overlaps with FOX3/NeuN staining in the perikarya of neurons. |
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| Chromogenic immunostaining of a formalin fixed paraffin embedded rat hippocampus section with mouse mAb to FOX3/NeuN, 1mg/mL 2083, dilution 1:2,000, detected in DAB (brown) following the Vector Labs ImmPRESS method with citra retrieval. Hematoxylin (blue) was used as the counterstain. This antibody has also been validated on NBF fixed human tissues.We used the data shown below to derive dissociation equilibrium constants (KD values) for specified mouse monoclonal antibodies. The KD is simply the ratio of the dissociation rate (koff) to the association rate (kon). Thus, KD and affinity are inversely related so that the lower the KD value, the higher the affinity of the antibody for its target. The figures below summarize binding data for EnCor's mouse monoclonal antibody to FOX3/NeuN (2083) and Millipore's mouse monoclonal antibody to the same target (M8718377, clone A60). Experiments were performed in parallel under identical conditions. Note that EnCor's 2083 has a significantly higher affinity for FOX3 (KD = 3nM) versus Millipore's M8718377 (KD = 8nM). |
 |
| Above: Binding curve set for 2083 (25nM IgG) and limiting dilutions of recombinant FOX3 peptide (0-500nM) obtained using our in-house label-free bio-layer interferometry system (Octet RED96). Color-coded traces show sensorgram data normalized to baseline after subtraction of 0nM IgG signal from all channels. Traces with overlying fit lines in red indicate their inclusion in the global fit analysis used to derive kinetic parameters listed under the legend (R^2 - goodness of correlation between the fit and data; kon - association rate constant; koff - dissociation rate constant; KD = koff/kon - affinity constant/equilibrium dissociation constant; see EnCor's validation pipeline for more details). Mouse click on the image to get an enlarged view. |
 |
| Above: Binding curve set for the Millipore mouse monoclonal antibody M8718377 (25nM IgG) and limiting dilutions of recombinant FOX3 peptide (0-500nM) obtained using our in-house label-free bio-layer interferometry system (Octet RED96). Color-coded traces show sensorgram data normalized to baseline after subtraction of 0nM IgG signal from all channels. Traces with overlying fit lines in red indicate their inclusion in the global fit analysis used to derive kinetic parameters listed under the legend (R^2 - goodness of correlation between the fit and data; kon - association rate constant; koff - dissociation rate constant; KD = koff/kon - affinity constant/equilibrium dissociation constant; see EnCor's validation pipeline for more details). Mouse click on the image to get an enlarged view. |
 |
| Western blot analysis of whole brain tissue lysates using mouse mAb to FOX3/NeuN 2083, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Note the strong twin bands corresponding to the two alternate transcripts of FOX3/NeuN protein with apparent SDS-PAGE molecular weights of 46 and 48kDa. As with other FOX3/NeuN antibodies, an additional band at ~70kDa is revealed in some lysates. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 497 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 37009224 | Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain | Suk TR, Nguyen TT, Fisk ZA, Mitkovski M, Geertsma HM, Parmasad JA, Heer MM, Callaghan SM, Benseler F, Brose N, Tirard M, Rousseaux MWC. | iScience. 2023 Mar 9;26(4):106350. doi: 10.1016/j.isci.2023.106350. eCollection 2023 Apr 21. |
| 36909498 | A New Behavioral Paradigm for Frustrative Non-reward Reveals a Global Change in Brain Networks by Frustration | Naik AA, Ma X, Munyeshyaka M, Leibenluft E, Li Z. | bioRxiv [Preprint]. 2023 Jun 6:2023.02.28.530477. doi: 10.1101/2023.02.28.530477. |
| 36630309 | Shared and Distinct Brain Regions Targeted for Immediate Early Gene Expression by Ketamine and Psilocybin | Davoudian PA, Shao LX, Kwan AC. | ACS Chem Neurosci. 2023 Feb 1;14(3):468-480. doi: 10.1021/acschemneuro.2c00637. Epub 2023 Jan 11. |
| 36625203 | Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in Tspan7 knockout rats | Pang S, Luo Z, Dong W, Gao S, Chen W, Liu N, Zhang X, Gao X, Li J, Gao K, Shi X, Guan F, Zhang L, Zhang L. | Life Sci Alliance. 2022 Dec 20;6(3):e202201616. doi: 10.26508/lsa.202201616. Print 2023 Mar. |
| 36618785 | Electroacupuncture attenuates spared nerve injury-induced neuropathic pain possibly by promoting the progression of AMPK/mTOR-mediated autophagy in spinal microglia | Cui Y, Hu C, Niu C, He M, Qiu X, Yao Q, Tian W, Xu Q. | Ann Transl Med. 2022 Dec;10(23):1278. doi: 10.21037/atm-22-5273. |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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|---|---|---|---|
| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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