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mouse anti-Mre11 monoclonal antibody (12D7) 9138

$503.00

Antibody summary

  • Mouse monoclonal to Mre11
  • Suitable for: WB,ICC/IF,IHC-P,IP,ELISA,Functional Assay,PLA
  • Isotype: IgG1
  • 100 µg
SKU: 9138parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Mre11

available sizes

100 µg

mouse anti-Mre11 monoclonal antibody (12D7) 9138

antibody
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
Immunoblotting: Use at 0.5-2ug/mL. A band of ~80kDa is detected. Detection of Mre11 in 30ug of whole cell extracts.

Immunofluorescence: use at 1-5ug/mL. Detection of Mre11 in HeLa cells fixed in 4% paraformaldehyde for 15 minutes.

These are recommended concentrations.

Endu
Immunogen
GST fusion protein corresponding to aa 182-582 of Mre11.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Double-strand break repair protein MRE11 (EC 3.1.-.-) (Double-strand break repair protein MRE11A) (Meiotic recombination 11 homolog 1) (MRE11 homolog 1) (Meiotic recombination 11 homolog A) (MRE11 homolog A)
Gene names
MRE11,MRE11 HNGS1 MRE11A
Protein family
MRE11/RAD32 family
Mass
80593Da
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11 (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). RAD50 may be required to bind DNA ends and hold them in close proximity (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289, PubMed:30612738). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
Subellular location
Nucleus. Chromosome, telomere. Chromosome. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.
Structure
Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:10839544, PubMed:26215093). As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites (PubMed:26215093). Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN (PubMed:10783165). Found in a complex with TERF2 (PubMed:10888888). Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo (PubMed:15456891, PubMed:15723659, PubMed:18469862). Interacts with ATF2 (PubMed:15916964). Interacts with EXD2 (PubMed:26807646). Interacts with MRNIP (PubMed:27568553). Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity (PubMed:28834754, PubMed:29670289). Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain) (PubMed:30612738). Interacts with CYREN (via XLF motif) (By similarity).; (Microbial infection) Interacts with herpes simplex virus 1 protein UL12 (PubMed:20943970).
Post-translational modification
Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.
Involvement in disease
DISEASE: Ataxia-telangiectasia-like disorder 1 (ATLD1) [MIM:604391]: A rare disorder characterized by progressive cerebellar ataxia, dysarthria, abnormal eye movements, and absence of telangiectasia. ATLD patients show normal levels of total IgG, IgA and IgM, although there may be reduced levels of specific functional antibodies. At the cellular level, ATLD exhibits hypersensitivity to ionizing radiation and radioresistant DNA synthesis. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Note=Defects in MRE11 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. A homozygous truncating mutation MRE11 has been found in patients with cerebellar vermis hypoplasia, ataxia and dysarthria.
Target Relevance information above includes information from UniProt accession: P49959
The UniProt Consortium

benchmark-antibodies_anti-mre11_antibody_9138_1.jpg
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (9138) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
benchmark-antibodies_anti-mre11_antibody_9138_2.jpg
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg whole cell extract of human Mre11-transfected 293T cells
7.5% SDS-PAGE
Mre11 antibody [12D7] (9138) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-mre11_antibody_9138_3.jpg
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (9138) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-mre11_antibody_9138_4.jpg
Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 stained by Mre11 antibody [12D7] (9138) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10 um.
benchmark-antibodies_anti-mre11_antibody_9138_5.jpg
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (9138) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-mre11_antibody_9138_6.jpg
Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
Mre11 stained by Mre11 antibody [12D7] (9138) diluted at 1:300.
Antigen Retrieval: EDTA buffer, 20 min

Publications

pmidtitleauthorscitation
38180818USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stressNusawardhana A, Pale LM, Nicolae CM, Moldovan GL.Nucleic Acids Res. 2024 Jan 5:gkad1237. doi: 10.1093/nar/gkad1237. Online ahead of print.
38177310YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent mannerZhang C, Chen L, Xie C, Wang F, Wang J, Zhou H, Liu Q, Zeng Z, Li N, Huang J, Zhao Y, Liu H.EMBO J. 2024 Jan;43(1):61-86. doi: 10.1038/s44318-023-00003-2. Epub 2023 Dec 15.
37805499Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleasesHale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL.Nat Commun. 2023 Oct 7;14(1):6265. doi: 10.1038/s41467-023-42011-0.
37486931Proliferating cell nuclear antigen inhibitors block distinct stages of herpes simplex virus infectionPackard JE, Williams MR, Fromuth DP, Dembowski JA.PLoS Pathog. 2023 Jul 24;19(7):e1011539. doi: 10.1371/journal.ppat.1011539. eCollection 2023 Jul.
37316668Mitotic tethering enables inheritance of shattered micronuclear chromosomesTrivedi P, Steele CD, Au FKC, Alexandrov LB, Cleveland DW.Nature. 2023 Jun;618(7967):1049-1056. doi: 10.1038/s41586-023-06216-z. Epub 2023 Jun 14.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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