Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | mouse |
isotype | IgG1 |
clonality | monoclonal |
concentration | 1 mg/mL |
applications | ICC/IF, WB |
reactivity | Mre11 |
available sizes | 100 µg |
mouse anti-Mre11 monoclonal antibody (12D7) 9138
$503.00
Antibody summary
- Mouse monoclonal to Mre11
- Suitable for: WB,ICC/IF,IHC-P,IP,ELISA,Functional Assay,PLA
- Isotype: IgG1
- 100 µg
mouse anti-Mre11 monoclonal antibody (12D7) 9138
antibody |
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Tested applications WB,IHC,IHC,ICC/IF |
Recommended dilutions Immunoblotting: Use at 0.5-2ug/mL. A band of ~80kDa is detected. Detection of Mre11 in 30ug of whole cell extracts. Immunofluorescence: use at 1-5ug/mL. Detection of Mre11 in HeLa cells fixed in 4% paraformaldehyde for 15 minutes. These are recommended concentrations. Endu |
Immunogen GST fusion protein corresponding to aa 182-582 of Mre11. |
Size and concentration 100µg and lot specific |
Form liquid |
Storage Instructions This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles. |
Storage buffer PBS, pH 7.4. |
Purity protein affinty purification |
Clonality monoclonal |
Isotype IgG1 |
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
Isotype control Mouse monocolonal IgG1 - Isotype Control |
target relevance |
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Protein names Double-strand break repair protein MRE11 (EC 3.1.-.-) (Double-strand break repair protein MRE11A) (Meiotic recombination 11 homolog 1) (MRE11 homolog 1) (Meiotic recombination 11 homolog A) (MRE11 homolog A) |
Gene names MRE11,MRE11 HNGS1 MRE11A |
Protein family MRE11/RAD32 family |
Mass 80593Da |
Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11 (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). RAD50 may be required to bind DNA ends and hold them in close proximity (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289, PubMed:30612738). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). |
Subellular location Nucleus. Chromosome, telomere. Chromosome. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. |
Structure Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:10839544, PubMed:26215093). As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites (PubMed:26215093). Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN (PubMed:10783165). Found in a complex with TERF2 (PubMed:10888888). Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo (PubMed:15456891, PubMed:15723659, PubMed:18469862). Interacts with ATF2 (PubMed:15916964). Interacts with EXD2 (PubMed:26807646). Interacts with MRNIP (PubMed:27568553). Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity (PubMed:28834754, PubMed:29670289). Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain) (PubMed:30612738). Interacts with CYREN (via XLF motif) (By similarity).; (Microbial infection) Interacts with herpes simplex virus 1 protein UL12 (PubMed:20943970). |
Post-translational modification Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome. |
Involvement in disease DISEASE: Ataxia-telangiectasia-like disorder 1 (ATLD1) [MIM:604391]: A rare disorder characterized by progressive cerebellar ataxia, dysarthria, abnormal eye movements, and absence of telangiectasia. ATLD patients show normal levels of total IgG, IgA and IgM, although there may be reduced levels of specific functional antibodies. At the cellular level, ATLD exhibits hypersensitivity to ionizing radiation and radioresistant DNA synthesis. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Note=Defects in MRE11 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. A homozygous truncating mutation MRE11 has been found in patients with cerebellar vermis hypoplasia, ataxia and dysarthria. |
Target Relevance information above includes information from UniProt accession: P49959 |
The UniProt Consortium |
Publications
pmid | title | authors | citation |
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38180818 | USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress | Nusawardhana A, Pale LM, Nicolae CM, Moldovan GL. | Nucleic Acids Res. 2024 Jan 5:gkad1237. doi: 10.1093/nar/gkad1237. Online ahead of print. |
38177310 | YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner | Zhang C, Chen L, Xie C, Wang F, Wang J, Zhou H, Liu Q, Zeng Z, Li N, Huang J, Zhao Y, Liu H. | EMBO J. 2024 Jan;43(1):61-86. doi: 10.1038/s44318-023-00003-2. Epub 2023 Dec 15. |
37805499 | Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases | Hale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL. | Nat Commun. 2023 Oct 7;14(1):6265. doi: 10.1038/s41467-023-42011-0. |
37486931 | Proliferating cell nuclear antigen inhibitors block distinct stages of herpes simplex virus infection | Packard JE, Williams MR, Fromuth DP, Dembowski JA. | PLoS Pathog. 2023 Jul 24;19(7):e1011539. doi: 10.1371/journal.ppat.1011539. eCollection 2023 Jul. |
37316668 | Mitotic tethering enables inheritance of shattered micronuclear chromosomes | Trivedi P, Steele CD, Au FKC, Alexandrov LB, Cleveland DW. | Nature. 2023 Jun;618(7967):1049-1056. doi: 10.1038/s41586-023-06216-z. Epub 2023 Jun 14. |
relevant to this product |
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Western blot IHC ICC |
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