Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | mouse |
isotype | IgG2b |
clonality | monoclonal |
concentration | 1 mg/mL |
applications | ICC/IF, IHC, WB |
reactivity | human, mouse, rat |
available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-MAP2 monoclonal antibody (5H11) 1367
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to MAP2
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG2b
- 100 µg, 25 µg, 1 mg
mouse anti-MAP2 monoclonal antibody (5H11) 1367
antibody |
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Database link: human P11137 mouse P20357 rat P15146 |
Tested applications WB,IHC,IHC,ICC/IF |
Recommended dilutions WB: 1:10000 IF/ICC and IHC: 1:1000 |
Immunogen Full length purified bovine protein, epitope mapped to projection domain of human sequence, amino acids 1057-1588 using EnCor product Prot-r-MAP2-P3 |
Size and concentration 25, 100, 1000µg and 1 mg/mL |
Form liquid |
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Storage buffer PBS, 50% glycerol, 0.04% NaN3 |
Purity affinity purified |
Clonality monoclonal |
Isotype IgG2b |
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
Isotype control Mouse monocolonal IgG2b - Isotype Control |
target relevance |
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As a key component of the cytoskeleton in neurons, MAP2 plays a critical role in regulating microtubule dynamics and stabilizing neuronal processes such as dendrites and axons. Its abundant presence in these structures makes it an essential factor in establishing and maintaining the intricate neuronal morphology required for proper cell function and connectivity in the nervous system. Researchers have focused on studying MAP2 to gain deeper insights into neuronal development, synaptic plasticity, and neurodegenerative diseases, as its expression and phosphorylation are closely associated with synaptic maturation and neuronal plasticity. As a cell marker, MAP2 serves as an invaluable marker for identifying neuronal differentiation and assessing neuronal damage or degeneration. This antibody recognizes MAP2 in neuronal cells and can be used as a cell marker antibody when used with a suitable secondary antibody. Click for more on: cell markers and MAP2 |
Protein names Microtubule-associated protein 2 (MAP-2) |
Gene names MAP2,MAP2 |
Mass 199526Da |
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. |
Subellular location Cytoplasm, cytoskeleton Cell projection, dendrite |
Structure Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126). |
Post-translational modification Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). |
Target Relevance information above includes information from UniProt accession: P11137 |
The UniProt Consortium |
Data
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 9 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
pmid | title | authors | citation |
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22623668 | An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of Ranvier | Gasser A, Ho TS, Cheng X, Chang KJ, Waxman SG, Rasband MN, Dib-Hajj SD. | J Neurosci. 2012 May 23;32(21):7232-43. doi: 10.1523/JNEUROSCI.5434-11.2012. |
18988710 | Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiation | Walton NM, Snyder GE, Park D, Kobeissy F, Scheffler B, Steindler DA. | Stem Cells. 2009 Feb;27(2):280-9. doi: 10.1634/stemcells.2008-0842. |
16832065 | Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells | Goetz AK, Scheffler B, Chen HX, Wang S, Suslov O, Xiang H, Brüstle O, Roper SN, Steindler DA. | Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):11063-8. doi: 10.1073/pnas.0510926103. Epub 2006 Jul 10. |
16817858 | Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily | Rush AM, Wittmack EK, Tyrrell L, Black JA, Dib-Hajj SD, Waxman SG. | Eur J Neurosci. 2006 May;23(10):2551-62. doi: 10.1111/j.1460-9568.2006.04789.x. |
16487970 | Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor | Eckenstein FP, McGovern T, Kern D, Deignan J. | Exp Neurol. 2006 Apr;198(2):338-49. doi: 10.1016/j.expneurol.2005.12.020. Epub 2006 Feb 17. |
Protocols
relevant to this product |
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Western blot IHC ICC |
Documents
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