ICC/IF image mouse anti MAP2 monoclonal antibody 2C4 5291

mouse anti-MAP2 monoclonal antibody (2C4) 5291

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to MAP2
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

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SKU: 5291parent Categories: antibody, cell marker Tag: MAP2

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-MAP2 monoclonal antibody (2C4) 5291

antibody
Database link: human P11137 mouse P20357 rat P15146
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:5000-1:10000 IF/ICC and IHC: 1:5000-10000
Immunogen Full length recombinant human MAP2D protein Prot-r-MAP2D expressed in and purified from E. coli.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
As a key component of the cytoskeleton in neurons, MAP2 plays a critical role in regulating microtubule dynamics and stabilizing neuronal processes such as dendrites and axons. Its abundant presence in these structures makes it an essential factor in establishing and maintaining the intricate neuronal morphology required for proper cell function and connectivity in the nervous system. Researchers have focused on studying MAP2 to gain deeper insights into neuronal development, synaptic plasticity, and neurodegenerative diseases, as its expression and phosphorylation are closely associated with synaptic maturation and neuronal plasticity. As a cell marker, MAP2 serves as an invaluable marker for identifying neuronal differentiation and assessing neuronal damage or degeneration. This antibody recognizes MAP2 in neuronal cells and can be used as a cell marker antibody when used with a suitable secondary antibody. Click for more on: cell markers and MAP2
Protein names Microtubule-associated protein 2 (MAP-2)
Gene names MAP2,MAP2
Mass 199526Da
Function FUNCTION: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subellular location SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}. Cell projection, dendrite {ECO:0000250\|UniProtKB:P20357}.
Structure SUBUNIT: Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126). {ECO:0000250\|UniProtKB:P20357, ECO:0000269\|PubMed:33894126}.
Post-translational modification PTM: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). {ECO:0000250\|UniProtKB:P15146, ECO:0000250\|UniProtKB:P20357}.
Target Relevance information above includes information from UniProt accession: P11137
The UniProt Consortium

Data

Immunofluorescent analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, 5291, dilution 1:5,000 in green, and chicken pAb to FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. 5291 antibody labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites. The FOX2 antibody stains the nuclei of most neuronal cells.

Chromogenic immunostaining of a formalin fixed paraffin embedded human hippocampus section with mouse mAb to MAP2A/B/C/D, 5291, dilution 1:5,000, detected in DAB(brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In this image, the MAP2 antibody specifically labels neuronal cell bodies and projections. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues.

Western blot analysis of tissue and cell lysates using mouse mAb to MAP2C/D, 5291, dilution 1:5,000 in green, and chicken pAb to vimentin, 6886, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293 and [6] NIH-3T3 cell lysates. A band at about 280kDa is full length intact MAP2A/2B isotypes while bands at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. The 6886 antibody binds to a single band at ~50kDa present in all preparations.

Publications

pmid	title	authors	citation
22623668	An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of Ranvier.	Andreas Gasser, Tammy Szu-Yu Ho, Xiaoyang Cheng, Kae-Jiun Chang, Stephen G Waxman, Matthew N Rasband, Sulayman D Dib-Hajj	J Neurosci 32:7232-43
18988710	Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiation.	Noah M Walton, Gregory E Snyder, Donghyun Park, Firas Kobeissy, Bjorn Scheffler, Dennis A Steindler	Stem Cells 27:280-9
16832065	Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells.	A Katrin Goetz, Bjorn Scheffler, Huan-Xin Chen, Shanshan Wang, Oleg Suslov, Hui Xiang, Oliver Br�stle, Steve N Roper, Dennis A Steindler	Proc Natl Acad Sci U S A 103:11063-8
16817858	Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily.	Anthony M Rush, Ellen K Wittmack, Lynda Tyrrell, Joel A Black, Sulayman D Dib-Hajj, Stephen G Waxman	Eur J Neurosci 23:2551-62
16487970	Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor.	Felix P Eckenstein, Toby McGovern, Drew Kern, Jason Deignan	Exp Neurol 198:338-49

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.