mouse anti-LAMP1 monoclonal antibody (H4A3) 9022 - Image 2

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022 - Image 3

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022 - Image 4

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022 - Image 5

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022 - Image 6

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022 - Image 7

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022

$409.00

Antibody summary

Mouse monoclonal to LAMP1
Suitable for: WB, ICC/IF, IHC
Reacts with: human
Isotype: IgG1
100 µg

SKU: 9022 Categories: antibody, cell marker Tag: LAMP1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
available sizes	100 µg

mouse anti-LAMP1 monoclonal antibody (H4A3) 9022

antibody
Database link: human P11279
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:10000 IF/ICC and IHC: 1:2000
Immunogen Amino acids 32-350 of the human LAMP1 precursor sequence in NP_005552.3 purified from E. coli
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Lysosomal-associated membrane protein 1 (LAMP1) serves as an essential cell marker in various fields of research, particularly in cell biology and immunology. LAMP1 is a glycoprotein localized to lysosomal membranes and late endosomes, playing a critical role in maintaining lysosomal integrity and facilitating endocytic processes. Due to its specific subcellular localization, LAMP1 is used as a marker to identify lysosomes and late endosomes in different cell types and tissues. Immunofluorescence and immunohistochemistry techniques, employing antibodies targeting LAMP1, enable researchers to visualize and study lysosomal compartments and their dynamics. Furthermore, LAMP1 is valuable in investigating lysosomal-related diseases, autophagy, and vesicle trafficking. In immunology, LAMP1 has gained importance as a marker for dendritic cell maturation and activation, aiding in the study of antigen presentation and immune response regulation. Click for more on: cell markers and Lamp1
Protein names Lysosome-associated membrane glycoprotein 1 (LAMP-1) (Lysosome-associated membrane protein 1) (CD107 antigen-like family member A) (CD antigen CD107a)
Gene names LAMP1,LAMP1
Protein family LAMP family
Mass 44882Da
Function Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, autophagy, and cholesterol homeostasis (By similarity). Also plays an important role in NK-cells cytotoxicity (PubMed:23632890, PubMed:2022921). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. ; (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). ; (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Subellular location Cell membrane Endosome membrane; Single-pass type I membrane protein Lysosome membrane Late endosome membrane Cytolytic granule membrane Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome (PubMed:16176980).
Post-translational modification O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.
Target Relevance information above includes information from UniProt accession: P11279
The UniProt Consortium

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LAMP-1 Antibody (H4A3) Alexa Fluor® 546:AF546. Direct fluorescent western blot analysis of LAMP-1 expression in U-937 (A) and ECV304 (B) whole cell lysates.

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LAMP-1 Antibody (H4A3): Western blot analysis of LAMP-1 expression in HeLa (A), JAR (B), ECV304 (C), U-937 (D), Jurkat (E) and Ramos (F) whole cell lysates.

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LAMP-1 Antibody (H4A3): Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.

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LAMP-1 Antibody (H4A3): Near-infrared western blot analysis of LAMP-1 expression in MDA-MB-231 (A), K-562 (B), HT-1080 (C) and JAR (D) whole cell lysates. Detection reagent used: m-IgGκ BP-CFL 680.

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LAMP-1 Antibody (H4A3): Near-infrared western blot analysis of LAMP-1 expression in MDA-MB-231 (A), K-562 (B), HT-1080 (C) and JAR (D) whole cell lysates.

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LAMP-1 Antibody (H4A3) Alexa Fluor® 488. Direct fluorescent western blot analysis of LAMP-1 expression in U-937 (A), ECV304 (B) and Jurkat (C) whole cell lysates.

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Simultaneous direct near-infrared western blot analysis of LAMP-1 expression, detected with LAMP-1 Antibody (H4A3) Alexa Fluor® 680: AF680 and β-Actin expression, detected with β-Actin (C4) Alexa Fluor® 790: AF790 in HT-1080 (A) and HeLa (B) whole cell lysates.

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LAMP-1 Antibody (H4A3) PE. FCM analysis of JAR cells. Black line histogram represents the isotype control, normal mouse IgG1.

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Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.