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mouse anti-Id3 monoclonal antibody (2B11) 1683

$503.00

Antibody summary

  • Mouse monoclonal to Id3
  • Suitable for: WB,ICC/IF,IP
  • Isotype: IgG1
  • 100 µg
SKU: 1683parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Id3

available sizes

100 µg

mouse anti-Id3 monoclonal antibody (2B11) 1683

antibody
Tested applications
WB,ICC/IF,IP
Recommended dilutions
Immunoblotting and Immuno- precipitation: use at 1-5 ug/mL.

Positive controls: Id3 transfected Sol8 muscles cells and recombinant fusion protein.
Immunogen
Mouse Id3 gst-fusion protein (aa 1-119) expressed in E. coli.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Storage buffer
10 mM PBS, pH 7.4.
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
DNA-binding protein inhibitor ID-3 (Class B basic helix-loop-helix protein 25) (bHLHb25) (Helix-loop-helix protein HEIR-1) (ID-like protein inhibitor HLH 1R21) (Inhibitor of DNA binding 3) (Inhibitor of differentiation 3)
Gene names
ID3,ID3 1R21 BHLHB25 HEIR1
Mass
12999Da
Function
FUNCTION: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. {ECO:0000269|PubMed:8437843}.
Subellular location
SUBCELLULAR LOCATION: Nucleus.
Tissues
TISSUE SPECIFICITY: Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Structure
SUBUNIT: Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation (By similarity). Interacts with CLOCK and BMAL1. {ECO:0000250, ECO:0000269|PubMed:20861012}.
Target Relevance information above includes information from UniProt accession : Q02535
The UniProt Consortium

Data

benchmark-antibodies_anti-id3_antibody_1683_1.jpg
Id3 antibody [2B11] detects Id3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Id3 protein stained by Id3 antibody [2B11] (1683) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
benchmark-antibodies_anti-id3_antibody_1683_2.jpg
Non-transfected (?) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] (1683) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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