mouse anti-Human Platelet GMP-140 (P-selectin/CD62) monoclonal antibody (WGA-1) 7586

$586.00

Antibody summary

Mouse monoclonal to Human Platelet GMP-140 (P-selectin/CD62)
Suitable for: WB,IHC,IP,FACS,FC
Isotype: IgG1
100 µg

SKU: 7586parent Category: antibody Tags: monoclonal, mouse, Platelet GMP-140 (P-selectin/CD62)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	Platelet GMP-140 (P-selectin/CD62)
available sizes	100 µg

mouse anti-Human Platelet GMP-140 (P-selectin/CD62) monoclonal antibody (WGA-1) 7586

antibody

Tested applications WB,IHC,IHC,IP
Recommended dilutions ELISA: 1-10ug/ml with immobilized antigen.Flow cytometry: 1-10ug/mlWestern Blot: 5-10ug/ml, non-reducing and non-heating conditions. Immunohistochemistry: 5-10ug/ml, frozen tissue sections.
Immunogen Human platelets
Size and concentration 100µg and
Form lyophilized
Storage Instructions This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°
Storage buffer PBS, pH 7.4, 1% BSA, lyophilized.
Purity immunogen affinty purifcation
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Protein names P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P)
Gene names SELP,SELP GMRP GRMP
Protein family Selectin/LECAM family
Mass 90820Da
Function FUNCTION: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. {ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:28011641, ECO:0000269\|PubMed:7585950}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:28011641}; Single-pass type I membrane protein {ECO:0000305\|PubMed:28011641}.
Tissues TISSUE SPECIFICITY: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Structure SUBUNIT: Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. {ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:11237770, ECO:0000269\|PubMed:15769472, ECO:0000269\|PubMed:18606703, ECO:0000269\|PubMed:7559387, ECO:0000269\|PubMed:7585949, ECO:0000269\|PubMed:7585950}.
Domain TOPO_DOM 4
Target Relevance information above includes information from UniProt accession : P16109
The UniProt Consortium

Data

benchmark-antibodies_anti-platelet_gmp-140_p-selectin-cd62_antibody_758

Flow cytometry: 1-10ug/ml Thrombin-activated human platelets incubated 30 minutes at room temperature with 1-2ug #7586 per 107 cells/ml and FITC-labeled secondary antibody.

Western Blot: 5-10ug/ml, non-reducing and non-heating conditions. Immunohistochemistry: 5-10ug/ml, frozen tissue sections.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.