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mouse anti-Human Platelet GMP-140 (P-selectin/CD62) monoclonal antibody (WGA-1) 7586

$586.00

Antibody summary

  • Mouse monoclonal to Human Platelet GMP-140 (P-selectin/CD62)
  • Suitable for: WB,IHC,IP,FACS,FC
  • Isotype: IgG1
  • 100 µg
SKU: 7586parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Platelet GMP-140 (P-selectin/CD62)

available sizes

100 µg

Available product – mouse anti-Human Platelet GMP-140 (P-selectin/CD62) monoclonal antibody (WGA-1) 7586

antibody
Tested applications
WB,IHC,IHC,IP
Recommended dilutions
ELISA: 1-10ug/ml with immobilized antigen.Flow cytometry: 1-10ug/mlWestern Blot: 5-10ug/ml, non-reducing and non-heating conditions.

Immunohistochemistry: 5-10ug/ml, frozen tissue sections.
Immunogen
Human platelets
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°
Storage buffer
PBS, pH 7.4, 1% BSA, lyophilized.
Purity
immunogen affinty purifcation
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P)
Gene names
SELP,SELP GMRP GRMP
Protein family
Selectin/LECAM family
Mass
90820Da
Function
FUNCTION: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:28011641, ECO:0000269|PubMed:7585950}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28011641}; Single-pass type I membrane protein {ECO:0000305|PubMed:28011641}.
Tissues
TISSUE SPECIFICITY: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Structure
SUBUNIT: Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:11237770, ECO:0000269|PubMed:15769472, ECO:0000269|PubMed:18606703, ECO:0000269|PubMed:7559387, ECO:0000269|PubMed:7585949, ECO:0000269|PubMed:7585950}.
Domain
TOPO_DOM 4
Target Relevance information above includes information from UniProt accession : P16109
The UniProt Consortium

Data

benchmark-antibodies_anti-platelet_gmp-140_p-selectin-cd62_antibody_758
Flow cytometry: 1-10ug/ml Thrombin-activated human platelets incubated 30 minutes at room temperature with 1-2ug #7586 per 107 cells/ml and FITC-labeled secondary antibody.
benchmark-antibodies_anti-platelet_gmp-140_p-selectin-cd62_antibody_758
Western Blot: 5-10ug/ml, non-reducing and non-heating conditions. Immunohistochemistry: 5-10ug/ml, frozen tissue sections.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC

Documents

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