| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Platelet GMP-140 (P-selectin/CD62) |
| available sizes | 100 µg |
mouse anti-Human Platelet GMP-140 (P-selectin/CD62) monoclonal antibody (WGA-1) 7586
$586.00
Antibody summary
- Mouse monoclonal to Human Platelet GMP-140 (P-selectin/CD62)
- Suitable for: WB,IHC,IP,FACS,FC
- Isotype: IgG1
- 100 µg
mouse anti-Human Platelet GMP-140 (P-selectin/CD62) monoclonal antibody (WGA-1) 7586
| antibody |
|---|
| Tested applications WB,IHC,IHC,IP |
| Recommended dilutions ELISA: 1-10ug/ml with immobilized antigen.Flow cytometry: 1-10ug/mlWestern Blot: 5-10ug/ml, non-reducing and non-heating conditions. Immunohistochemistry: 5-10ug/ml, frozen tissue sections. |
| Immunogen Human platelets |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4° |
| Storage buffer PBS, pH 7.4, 1% BSA, lyophilized. |
| Purity immunogen affinity purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P) |
| Gene names SELP,SELP GMRP GRMP |
| Protein family Selectin/LECAM family |
| Mass 90820Da |
| Function FUNCTION: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3) (PubMed:37184585). {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:28011641, ECO:0000269|PubMed:37184585, ECO:0000269|PubMed:7585950}. |
| Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28011641}; Single-pass type I membrane protein {ECO:0000305|PubMed:28011641}. |
| Tissues TISSUE SPECIFICITY: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. |
| Structure SUBUNIT: Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. Interacts (via C-type lectin domain) with alpha-IIb/beta3 integrin ITGA2B:ITGB3 and alpha-V/beta-3 integrin ITGAV:ITGB3 (PubMed:37184585). Interacts with alpha5/beta1 integrin ITGA5:ITGB1 and alpha4/beta1 integrin ITGA4:ITGB (PubMed:37184585). {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:11237770, ECO:0000269|PubMed:15769472, ECO:0000269|PubMed:18606703, ECO:0000269|PubMed:37184585, ECO:0000269|PubMed:7559387, ECO:0000269|PubMed:7585949, ECO:0000269|PubMed:7585950}. |
| Domain DOMAIN: The C-type lectin domain is required for binding to integrins (PubMed:37184585). Binding to soluble integrins alpha-V/beta-3 (ITGAV:ITGB3) and alpha-IIb/beta3 (ITGA2B:ITGB) is cation-dependent in order of preference of 1 mM Mn(2+) > Mg(2+) > Ca(2+) (PubMed:37184585). Binds to the allosteric site (site 2) of integrins and activates them (PubMed:37184585). The interaction with integrins may mediate cell-cell interactions and cell adhesion (PubMed:37184585). {ECO:0000269|PubMed:37184585}. |
| Target Relevance information above includes information from UniProt accession: P16109 |
| The UniProt Consortium |
Data
 |
| Flow cytometry: 1-10ug/ml Thrombin-activated human platelets incubated 30 minutes at room temperature with 1-2ug #7586 per 107 cells/ml and FITC-labeled secondary antibody. |
 |
| Western Blot: 5-10ug/ml, non-reducing and non-heating conditions. Immunohistochemistry: 5-10ug/ml, frozen tissue sections. |
Publications
Publications
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| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
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| Western blot IHC |
Documents
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