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mouse anti-GFAP monoclonal antibody (2E1) 9024

$409.00

Antibody summary

  • Mouse monoclonal to GFAP
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG2b
  • 100 µg
SKU: 9024 Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

100 µg

mouse anti-GFAP monoclonal antibody (2E1) 9024

antibody
Database link:
human P14136
mouse P03995
rat P47819
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:5000 IF/ICC and IHC 1:1000-1,5000
Immunogen
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Size and concentration
100µL and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
serum
Clonality
monoclonal
Isotype
IgG2b
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG2b - Isotype Control
target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders.

Click for more on: cell markers and GFAP
Protein names
Glial fibrillary acidic protein (GFAP)
Gene names
GFAP,GFAP
Protein family
Intermediate filament family
Mass
49880Da
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location
Cytoplasm Note=Associated with intermediate filaments.
Tissues
Expressed in cells lacking fibronectin.
Structure
Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus).
Post-translational modification
Phosphorylated by PKN1.
Involvement in disease
Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14136
The UniProt Consortium

Data

benchmarkantibodies.com/wp-content/uploads/2024/03/9024-1.jpg
Western blot analysis of GFAP expression in C6 (A), U-87 MG (B), SK-N-SH (C) and U-251-MG (D) whole cell lysates. Detection reagent used: m-IgGκ BP-HRP.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-2.jpg
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of astrocytes and neuropil staining.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-3.jpg
Direct fluorescent western blot analysis of GFAP expression in U-251-MG whole cell lysate.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-4.jpg
Western blot analysis of GFAP expression in U-87 MG (A), U-251-MG (B), C2C12 (C) and Neuro-2A (D) whole cell lysates.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-5.jpg
Direct near-infrared western blot analysis of GFAP expression in human brain tissue extract.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-6.jpg
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain showing staining of astrocytes.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-7.jpg
Near-Infrared western blot analysis of GFAP expression in non-transfected 293T (A), human GFAP transfected 293T (B), C6 (C) and U-251-MG (D) whole cell lysates.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-8.jpg
Western blot analysis of GFAP expression in C6 whole cell lysate (A) and mouse brain tissue extract (B).
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-9.jpg
Near-Infrared western blot analysis of GFAP expression in non-transfected (A) human GFAP transfected 293T (B) and human GFAP transfected 293T (C) whole cell lysates.
benchmarkantibodies.com/wp-content/uploads/2024/03/9024-10.jpg
Western blot analysis of GFAP expression in rat brain tissue extract.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 30 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
37759938Neuroimmune Support of Neuronal Regeneration and Neuroplasticity following Cerebral Ischemia in Juvenile MiceMarquez-Ortiz RA, Tesic V, Hernandez DR, Akhter B, Aich N, Boudreaux PM, Clemons GA, Wu CY, Lin HW, Rodgers KM.Brain Sci. 2023 Sep 17;13(9):1337. doi: 10.3390/brainsci13091337.
37667307Metal-organic framework materials promote neural differentiation of dental pulp stem cells in spinal cord injuryZhou H, Jing S, Xiong W, Zhu Y, Duan X, Li R, Peng Y, Kumeria T, He Y, Ye Q.J Nanobiotechnology. 2023 Sep 4;21(1):316. doi: 10.1186/s12951-023-02001-2.
37605262Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouseCrivelli SM, Quadri Z, Vekaria HJ, Zhu Z, Tripathi P, Elsherbini A, Zhang L, Sullivan PG, Bieberich E.Acta Neuropathol Commun. 2023 Aug 21;11(1):135. doi: 10.1186/s40478-023-01633-7.
37587766USP25 Inhibits Neuroinflammatory Responses After Cerebral Ischemic Stroke by Deubiquitinating TAB2Li Z, Liu B, Lambertsen KL, Clausen BH, Zhu Z, Du X, Xu Y, Poulsen FR, Halle B, Bonde C, Chen M, Wang X, Schlüter D, Huang J, Waisman A, Song W, Wang X.Adv Sci (Weinh). 2023 Oct;10(28):e2301641. doi: 10.1002/advs.202301641. Epub 2023 Aug 16.
37513944EYE-503: A Novel Retinoic Acid Drug for Treating Retinal NeurodegenerationLiu S, Ji Y, Li H, Ren L, Zhu J, Yang T, Li X, Yao J, Cao X, Yan B.Pharmaceuticals (Basel). 2023 Jul 20;16(7):1033. doi: 10.3390/ph16071033.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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