| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-GFAP monoclonal antibody (2A5) 3682
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to GFAP
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG1
- 100 µg, 25 µg, 1 mg
mouse anti-GFAP monoclonal antibody (2A5) 3682
| target relevance |
|---|
| Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP |
| Protein names Glial fibrillary acidic protein (GFAP) |
| Gene names GFAP,GFAP |
| Protein family Intermediate filament family |
| Mass 49880Da |
| Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Subellular location Cytoplasm Note=Associated with intermediate filaments. |
| Tissues Expressed in cells lacking fibronectin. |
| Structure Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus). |
| Post-translational modification Phosphorylated by PKN1. |
| Involvement in disease Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: P14136 |
| The UniProt Consortium |
Data
 |
| Paraffin embedded histological section of human cerebellum stained with 3682 using the HRP/ DAB staining, counterstained with hematoxylin/eosin. Tissues were fixed in formalin and processed for paraffin embedding. To the top left is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The bottom right shows a region of the granular layer and to further to the right is white matter, both of which contain GFAP positive astrocytes. 5µ paraffin embedded section staining was achieved using a 15 minute pressure cooker heat retrieval in Abcam Antigen Retrieval Buffer, Citrate buffer at pH=6.0, and staining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit. See here for further details. |
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| Immunofluorescent analysis of an adult rat cerebellum section stained with mouse mAb to GFAP, 3682, dilution 1:500, in red, and costained with chicken pAb to parvalbumin, , dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45µM sections were stained with above antibodies. The GFAP antibody stains the processes of Bergmann glia and astrocytes, though is not optimal for this purpose, likely because the epitope is slightly variant in rodent compared to the human and the pig sequence. For routine staining of GFAP we recommend other EnCor products, a different mouse monoclonal 3743 to GFAP or one of our popular GFAP polyclonals, 2539, 6048 and 8205. The antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material. Mouse select for larger image. |
 |
| Western blot analysis of equal amount of total protein from different tissue lysates and recombinant proteins solutions using mouse mAb to GFAP, 3682, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] rat recombinant GFAP, [8] human recombinant GFAP. Bands around 50kDa correspond to alternative transcripts and proteolytic products of GFAP. Note that 3682 antibody has significantly stronger reactivity with pig and human GFAP as compared to rodent, suggesting that it binds to an epitope which is not totally conserved across mammalian sequences. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 76 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36419095 | Novel localization of folate transport systems in the murine central nervous system | Sangha V, Hoque MT, Henderson JT, Bendayan R. | Fluids Barriers CNS. 2022 Nov 23;19(1):92. doi: 10.1186/s12987-022-00391-3. |
| 36160643 | Ji-Chuan decoction ameliorates slow transit constipation via regulation of intestinal glial cell apoptosis | Wang XM, Lv LX, Qin YS, Zhang YZ, Yang N, Wu S, Xia XW, Yang H, Xu H, Liu Y, Ding WJ. | World J Gastroenterol. 2022 Sep 14;28(34):5007-5022. doi: 10.3748/wjg.v28.i34.5007. |
| 35850708 | Expression and activation of nuclear hormone receptors result in neuronal differentiation and favorable prognosis in neuroblastoma | Sainero-Alcolado L, Mushtaq M, Liaño-Pons J, Rodriguez-Garcia A, Yuan Y, Liu T, Ruiz-Pérez MV, Schlisio S, Bedoya-Reina O, Arsenian-Henriksson M. | J Exp Clin Cancer Res. 2022 Jul 19;41(1):226. doi: 10.1186/s13046-022-02399-x. |
| 35808977 | Characterisation of the consequences of maternal immune activation on distinct cell populations in the developing rat spinal cord | Anderson RC, O'Keeffe GW, McDermott KW. | J Anat. 2022 Oct;241(4):938-950. doi: 10.1111/joa.13726. Epub 2022 Jul 9. |
| 35778934 | The LncRNA AK018453 regulates TRAP1/Smad signaling in IL-17-activated astrocytes: A potential role in EAE pathogenesis | Zhang Q, Yang Y, Chen Y, Wang Y, Qin S, Lv R, Zhou M, Yu Q, Li X, Li X, Wang X, You H, Wang Y, Zhou F, Liu X. | Glia. 2022 Nov;70(11):2079-2092. doi: 10.1002/glia.24239. Epub 2022 Jul 2. |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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|---|---|---|---|
| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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