| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Excitatory Amino Acid Transporter 1 |
| available sizes | 100 µg |
mouse anti-Excitatory Amino Acid Transporter 1 monoclonal antibody (2G7C2) 8730
$520.00
Antibody summary
- Mouse monoclonal to Excitatory Amino Acid Transporter 1
- Suitable for: WB,IHC,ELISA
- Isotype: IgG1
- 100 µg
mouse anti-Excitatory Amino Acid Transporter 1 monoclonal antibody (2G7C2) 8730
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions ELISA: use at 0.1-2ug/ml Immunoblotting: User should use at 1-5ug/ml. A band of ~60kDa is detected. Immunohistochemistry: Use at 2- 10ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide derived from the C-terminal domain of rat EAAT1. Accession no. P24942. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinity purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Excitatory amino acid transporter 1 (Glial glutamate transporter) (Sodium-dependent glutamate/aspartate transporter 1) (GLAST) (GLAST-1) (Solute carrier family 1 member 3) |
| Gene names Slc1a3,Slc1a3 Eaat1 |
| Protein family Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A3 subfamily |
| Mass 59697Da |
| Function FUNCTION: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:1279699, PubMed:8387171). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). {ECO:0000250|UniProtKB:O57321, ECO:0000250|UniProtKB:P56564, ECO:0000269|PubMed:1279699, ECO:0000269|PubMed:8387171}. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in); Xref=Rhea:RHEA:70699, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:29103, ChEBI:CHEBI:29985; Evidence={ECO:0000250|UniProtKB:P43003}; CATALYTIC ACTIVITY: Reaction=K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in); Xref=Rhea:RHEA:70851, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:29103, ChEBI:CHEBI:29991; Evidence={ECO:0000250|UniProtKB:P43003}; CATALYTIC ACTIVITY: Reaction=D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in); Xref=Rhea:RHEA:71379, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:29103, ChEBI:CHEBI:29990; Evidence={ECO:0000250|UniProtKB:P43003}; |
| Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1279699, ECO:0000269|PubMed:8387171}; Multi-pass membrane protein. |
| Tissues TISSUE SPECIFICITY: Detected in brain and cerebellum (PubMed:1279699, PubMed:8387171). Both isoform GLAST-1 and GLAST-1A are expressed in bone and brain (PubMed:11086157). In brain isoform GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed:11086157). {ECO:0000269|PubMed:11086157, ECO:0000269|PubMed:1279699, ECO:0000269|PubMed:8387171}. |
| Structure SUBUNIT: Homotrimer (By similarity). {ECO:0000250|UniProtKB:P43003}. |
| Post-translational modification PTM: Glycosylated. {ECO:0000269|PubMed:8521863}. |
| Domain DOMAIN: Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move. {ECO:0000250|UniProtKB:P43003}. |
| Target Relevance information above includes information from UniProt accession: P24942 |
| The UniProt Consortium |
Data
| No results found |
Publications
Publications
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Protocols
| relevant to this product |
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| Western blot IHC |
Documents
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