ICC/IF image mouse anti Doublecortin monoclonal antibody 3E1 8001

mouse anti-Doublecortin monoclonal antibody (3E1) 8001

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Doublecortin
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG2a
100 µg, 25 µg, 1 mg

SKU: 8001parent Categories: antibody, cell marker Tag: Doublecortin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Doublecortin monoclonal antibody (3E1) 8001

antibody
Database link: human O43602 mouse O88809 rat Q9ESI7
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:1000 IF/ICC and IHC: 1:1000
Immunogen Recombinant full length human Lis-A isoform of doublecortin expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2a - Isotype Control

target relevance
Doublecortin (DCX) is a widely used cell marker in neuroscience and developmental biology research. It is a microtubule-associated protein that plays a critical role in neuronal migration and maturation, particularly in the developing brain. DCX is selectively expressed in migrating neuroblasts and immature neurons, making it a valuable marker for identifying and studying neurogenesis and neuronal migration processes. Immunohistochemistry and immunofluorescence techniques utilizing antibodies targeting DCX enable researchers to visualize and quantify these migrating neuroblasts and young neurons in the brain and other tissues. Moreover, DCX has been employed in studies related to neuroplasticity and adult neurogenesis in the hippocampus, providing valuable insights into learning and memory processes in the adult brain. Click for more on: cell markers and Doubelcortin
Protein names Neuronal migration protein doublecortin (Doublin) (Lissencephalin-X) (Lis-X)
Gene names DCX,DCX DBCN LISX
Mass 40574Da
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Subellular location Cytoplasm Cell projection, neuron projection Note=Localizes at neurite tips.
Tissues Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Structure Interacts with tubulin (PubMed:27292316). Interacts with USP9X (PubMed:24607389).
Post-translational modification Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity). ; Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Involvement in disease Lissencephaly, X-linked 1 (LISX1) [MIM:300067]: A classic lissencephaly characterized by intellectual disability and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'. Note=The disease is caused by variants affecting the gene represented in this entry.; Subcortical band heterotopia X-linked (SBHX) [MIM:300067]: SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal. Note=The disease is caused by variants affecting the gene represented in this entry.; Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
Target Relevance information above includes information from UniProt accession: O43602
The UniProt Consortium

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to doublecortin, 8001, dilution 1:1,000 in red, and costained with chicken pAb to microtubule associated protein 2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons. Doublecortin antibody is an excellent marker of early developing neuronal cells.

Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat testes section with mouse mAb to doublecortin, 8001, dilution 1:1,000, ddetected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 8001 labels the Sertoli cells, the basement membrane and the late-stage spermatocytes. This antibody has not been tested in NBF fixed material.

Western blot analysis of rat whole brain lysates using mouse mAb to doublecortin, 8001, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40kDa and 45kDa correspond to the doublecortin protein, detected only in the developing brain.

Publications

pmid	title	authors	citation
28701917	Distinct Features of Doublecortin as a Marker of Neuronal Migration and Its Implications in Cancer Cell Mobility.	Abiola A Ayanlaja, Ye Xiong, Yue Gao, GuangQuan Ji, Chuanxi Tang, Zamzam Abdikani Abdullah, DianShuai Gao	Front Mol Neurosci 10:199
26498952	TH and DCX mRNAs in peripheral blood and bone marrow predict outcome in metastatic neuroblastoma patients.	Yania Yáñez, David Hervás, Elena Grau, Silvestre Oltra, Gema Pérez, Sarai Palanca, Mar Bermúdez, Catalina Márquez, Adela Cañete, Victoria Castel	J Cancer Res Clin Oncol 142:573-80
24590653	Neuroblastoma mRNAs predict outcome in children with stage 4 neuroblastoma: a European HR-NBL1/SIOPEN study.	Virginie F Viprey, Walter M Gregory, Maria V Corrias, Andrei Tchirkov, Katrien Swerts, Ales Vicha, Sandro Dallorso, Penelope Brock, Roberto Luksch, Dominique Valteau-Couanet, Vassilios Papadakis, Genevieve Laureys, Andrew D Pearson, Ruth Ladenstein, Susan A Burchill	J Clin Oncol 32:1074-83
20715151	JNK phosphorylates Ser332 of doublecortin and regulates its function in neurite extension and neuronal migration.	Junghee Jin, Hiromi Suzuki, Syu-Ichi Hirai, Katsuhiko Mikoshiba, Toshio Ohshima	Dev Neurobiol 70:929-42
15899794	Gene expression profiling and genetic markers in glioblastoma survival.	Jeremy N Rich, Christopher Hans, Beatrix Jones, Edwin S Iversen, Roger E McLendon, B K Ahmed Rasheed, Adrian Dobra, Holly K Dressman, Darell D Bigner, Joseph R Nevins, Mike West	Cancer Res 65:4051-8

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.