Immunohistochemistry (IHC) : Human basal cell carcinoma stained with anti-keratin 8 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

mouse anti-Cytokeratin 8 monoclonal antibody (ZM123) 6151

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Cytokeratin 8
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Breast carcinoma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6151parent Category: antibody Tags: anatomy, Cytokeratin 8, General & Epithelial Markers

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Cytokeratin 8 monoclonal antibody ZM123 6151

antibody
Database link: human P05787
Tested applications IHC
Recommended dilutions As directed
Immunogen Recombinant full-length human KRT8 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8)
Protein family Intermediate filament family
Mass 53704Da
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .
Subellular location Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
Tissues Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. .
Structure Heterotetramer of two type I and two type II keratins (PubMed:16000376). Forms a heterodimer with KRT18 (PubMed:10852826, PubMed:24940650). Associates with KRT20 (PubMed:10973561, PubMed:16608857). Interacts with PLEC isoform 1C, when in a heterodimer with KRT18 (PubMed:24940650). Interacts with PNN (PubMed:10809736). When associated with KRT19, interacts with DMD. Interacts with TCHP (PubMed:15731013). Interacts with APEX1 (PubMed:19188445). Interacts with GPER1 (PubMed:21149639). Interacts with EPPK1 (By similarity). Interacts with PKP1 and PKP2 (PubMed:10852826). .; (Microbial infection) Interacts with hepatitis C virus/HCV core protein. .
Post-translational modification PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. .; PTM: O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.; PTM: O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Domain DOMAIN 91..402; /note="IF rod"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01188"
Involvement in disease Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension. . Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P05787
The UniProt Consortium

Data

Human basal cell carcinoma stained with anti-keratin 8 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.