Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human Ewing’s sarcoma stained with clone ZM139 antibody using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of tumor cells

mouse anti-CD99 monoclonal antibody (ZM139) 6117

$160.00 – $528.00

Antibody summary

Mouse monoclonal to CD99
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Ewing’s sarcoma
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6117parent Category: antibody Tags: anatomy, CD99, Soft Tissue Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CD99 monoclonal antibody ZM139 6117

antibody
Database link: human P14209
Tested applications IHC
Recommended dilutions Concentrated 1:50-100
Applicaiton Notes Positive control: Ewing's sarcoma
Immunogen Purified E-rosette forming cells from human peripheral blood lymphocytes
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names CD99 antigen (12E7) (E2 antigen) (Protein MIC2) (T-cell surface glycoprotein E2) (CD antigen CD99)
Protein family CD99 family
Mass 18848Da
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Subellular location Membrane ; Single-pass type I membrane protein .
Post-translational modification PTM: Extensively O-glycosylated.
Target Relevance information above includes information from UniProt accession: P14209
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with clone ZM139 antibody using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of tumor cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.