Immunohistochemistry (IHC) : Human breast ductal carcinoma in situ stained with anti-Calponin-1 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of myoepithelial cells.

mouse anti-Calponin-1 monoclonal antibody (ZM21) 6049

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Calponin-1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Normal breast tissue
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6049parent Category: antibody Tags: anatomy, Calponin-1, Soft Tissue Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Calponin-1 monoclonal antibody ZM21 6049

antibody
Database link: human P51911
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Normal breast tissue
Immunogen Recombinant human CNN1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Calponin-1 (Basic calponin) (Calponin H1, smooth muscle)
Protein family Calponin family
Mass 33170Da
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Tissues Smooth muscle, and tissues containing significant amounts of smooth muscle.
Structure Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.
Domain DOMAIN 28..131; /note="Calponin-homology (CH)"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU00044"
Target Relevance information above includes information from UniProt accession: P51911
The UniProt Consortium

Data

Human breast ductal carcinoma in situ stained with anti-Calponin-1 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of myoepithelial cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.