| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-NeuN polyclonal antibody 1867
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to NeuN
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-NeuN polyclonal antibody 1867
| target relevance |
|---|
| NeuN, also known as Fox-3 or RBFOX3, is a well-established neuronal marker widely used in neuroscience research. It is a nuclear protein that is selectively expressed in post-mitotic neurons, making it an essential tool for identifying and characterizing neuronal populations in various tissues and experimental models. NeuN immunohistochemistry, facilitated by specific antibodies targeting NeuN, allows researchers to visualize and quantify neuronal cell bodies, providing valuable insights into neuronal distribution, density, and morphology. This marker is particularly valuable for studying neurodevelopment, neurodegenerative diseases, and neuronal plasticity in response to different experimental conditions or treatments. Moreover, NeuN antibodies have proven instrumental in identifying and differentiating neurons from other cell types, such as glial cells, in complex neural tissues. Click for more on: cell markers and NeuN |
| Protein names RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen) |
| Gene names RBFOX3,RBFOX3 |
| Mass 33873Da |
| Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). |
| Subellular location Nucleus Cytoplasm Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment. |
| Target Relevance information above includes information from UniProt accession : A6NFN3 |
| The UniProt Consortium |
Data
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| Immunofluorescent analysis of rat hippocampus section stained with chicken pAb to FOX3/NeuN, 1867, dilution 1:5,000 in red, and costained with rabbit pAb to IBA1 dilution 1:2,000, in green. Following transcardial perfusion with 4% paraformaldehyde, rat brain was post fixed for 24 hours, cut to 35µM, and free-floating sections were stained with the above antibodies. The FOX3/NeuN antibody stains the nuclei and distal perikarya of most neurons, while the IBA1 antibody specifically labels microglial cells. |
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| Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to FOX3/NeuN, 1867, dilution 1:10,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. FOX3/NeuN antibody stains the nuclei and distal perikaryal of most neurons. Mouse select image for larger view. |
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| Western blot analysis of mouse brain nuclear fraction lysate using chicken pAb to FOX3/NeuN, 1867, dilution 1:1,000 in green. Lane 1 represents protein standard in red. Two bands at 46 and 50kDa mark correspond to the two isoforms of the FOX3/NeuN protein. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 8 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36046623 | A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes | Agnew-Svoboda W, Ubina T, Figueroa Z, Wong YC, Vizcarra EA, Roebini B, Wilson EH, Fiacco TA, Riccomagno MM. | Cell Rep Methods. 2022 Aug 22;2(8):100276. doi: 10.1016/j.crmeth.2022.100276. eCollection 2022 Aug 22. |
| 21747913 | NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2 | Dredge BK, Jensen KB. | PLoS One. 2011;6(6):e21585. doi: 10.1371/journal.pone.0021585. Epub 2011 Jun 29. |
| 19713214 | Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors | Kim KK, Adelstein RS, Kawamoto S. | J Biol Chem. 2009 Nov 6;284(45):31052-61. doi: 10.1074/jbc.M109.052969. Epub 2009 Aug 27. |
| 19226510 | Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain | Azevedo FA, Carvalho LR, Grinberg LT, Farfel JM, Ferretti RE, Leite RE, Jacob Filho W, Lent R, Herculano-Houzel S. | J Comp Neurol. 2009 Apr 10;513(5):532-41. doi: 10.1002/cne.21974. |
| 16260614 | Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals | Underwood JG, Boutz PL, Dougherty JD, Stoilov P, Black DL. | Mol Cell Biol. 2005 Nov;25(22):10005-16. doi: 10.1128/MCB.25.22.10005-10016.2005. |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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