chicken anti-NeuN polyclonal antibody 1867

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to NeuN
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgY
100 µg, 25 µg, 1 mg

SKU: 1867parent Categories: antibody, cell marker Tag: NeuN

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

chicken anti-NeuN polyclonal antibody 1867

antibody
Database link: human A6NFN3 mouse Q8BIF2
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:500-1:1000 IF/IHC 1:5000-1:10000
Immunogen N-terminal 100 amino acids of human FOX3 expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
NeuN, also known as Fox-3 or RBFOX3, is a well-established neuronal marker widely used in neuroscience research. It is a nuclear protein that is selectively expressed in post-mitotic neurons, making it an essential tool for identifying and characterizing neuronal populations in various tissues and experimental models. NeuN immunohistochemistry, facilitated by specific antibodies targeting NeuN, allows researchers to visualize and quantify neuronal cell bodies, providing valuable insights into neuronal distribution, density, and morphology. This marker is particularly valuable for studying neurodevelopment, neurodegenerative diseases, and neuronal plasticity in response to different experimental conditions or treatments. Moreover, NeuN antibodies have proven instrumental in identifying and differentiating neurons from other cell types, such as glial cells, in complex neural tissues. Click for more on: cell markers and NeuN
Protein names RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen)
Gene names RBFOX3,RBFOX3
Mass 33873Da
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Subellular location Nucleus Cytoplasm Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment.
Target Relevance information above includes information from UniProt accession: A6NFN3
The UniProt Consortium

Immunofluorescent analysis of rat hippocampus section stained with chicken pAb to FOX3/NeuN, 1867, dilution 1:5,000 in red, and costained with rabbit pAb to IBA1 dilution 1:2,000, in green. Following transcardial perfusion with 4% paraformaldehyde, rat brain was post fixed for 24 hours, cut to 35µM, and free-floating sections were stained with the above antibodies. The FOX3/NeuN antibody stains the nuclei and distal perikarya of most neurons, while the IBA1 antibody specifically labels microglial cells.

Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to FOX3/NeuN, 1867, dilution 1:10,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. FOX3/NeuN antibody stains the nuclei and distal perikaryal of most neurons.

WB-image-chicken-anti-NeuN-polyclonal-antibody-1867

Western blot analysis of mouse brain nuclear fraction lysate using chicken pAb to FOX3/NeuN, 1867, dilution 1:1,000 in green. Lane 1 represents protein standard in red. Two bands at 46 and 50kDa mark correspond to the two isoforms of the FOX3/NeuN protein.

Publications

pmid	title	authors	citation
36046623	A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes.	William Agnew-Svoboda, Teresa Ubina, Zoe Figueroa, Yiu-Cheung Wong, Edward A Vizcarra, Bryan Roebini, Emma H Wilson, Todd A Fiacco, Martin M Riccomagno	Cell Rep Methods 2:100276
21747913	NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2.	B Kate Dredge, Kirk B Jensen	PLoS One 6:e21585
19713214	Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors.	Kee K Kim, Robert S Adelstein, Sachiyo Kawamoto	J Biol Chem 284:31052-61
19226510	Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain.	Frederico A C Azevedo, Ludmila R B Carvalho, Lea T Grinberg, José Marcelo Farfel, Renata E L Ferretti, Renata E P Leite, Wilson Jacob Filho, Roberto Lent, Suzana Herculano-Houzel	J Comp Neurol 513:532-41
16260614	Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals.	Jason G Underwood, Paul L Boutz, Joseph D Dougherty, Peter Stoilov, Douglas L Black	Mol Cell Biol 25:10005-16

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.