ICC/IF image chicken anti Ki67 polyclonal antibody 1247

chicken anti-Ki67 polyclonal antibody 1247

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to Ki67
Suitable for: WB, ICC/IF
Reacts with: human, mouse, rat
Isotype: IgY
100 µg, 25 µg, 1 mg

SKU: 1247parentz Categories: antibody, cell marker Tag: Ki-67

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

chicken anti-Ki67 polyclonal antibody 1247

antibody
Database link: human P46013 mouse E9PVX6 rat Q5RJM0
Tested applications WB,ICC/IF
Recommended dilutions WB: 1:5000 IF/ICC: 1:1000-5000
Immunogen Mixture of recombinant human ki-67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
ki-67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, ki-67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting ki-67 are commonly employed to visualize and quantify its expression in tissues and cell cultures. Click for more on: cell markers and ki-67
Protein names Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Gene names MKI67,MKI67
Mass 358694Da
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subellular location Chromosome Nucleus Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Structure Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126).
Post-translational modification Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Biotechnology Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).
Target Relevance information above includes information from UniProt accession: P46013
The UniProt Consortium

Data

Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, 1247, dilution 1:2,000 in red, and mouse mAb to beta-tubulin, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. The 1247 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state. The antibody produces strong staining of cytoplasmic microtubules.

Chromogenic immunostaining of a formalin fixed paraffin embedded murine small intestine cross section with chicken pAb to Ki67, 1247, dilution 1:10,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. Ki67 antibody specifically detects the nuclei of proliferating intestinal cells. Mouse select image at left for larger view.

Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, 1247, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67 is a rather unstable and short half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 12 publications in our database for this antibody or clone. Here are the latest 5, for more click below.

pmid	title	authors	citation
34433050	The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus	Gupta B, Errington AC, Jimenez-Pascual A, Eftychidis V, Brabletz S, Stemmler MP, Brabletz T, Petrik D, Siebzehnrubl FA.	Cell Rep. 2021 Aug 24;36(8):109588. doi: 10.1016/j.celrep.2021.109588.
34285243	TNFα increases tyrosine hydroxylase expression in human monocytes	Gopinath A, Badov M, Francis M, Shaw G, Collins A, Miller DR, Hansen CA, Mackie P, Tansey MG, Dagra A, Madorsky I, Ramirez-Zamora A, Okun MS, Streit WJ, Khoshbouei H.	NPJ Parkinsons Dis. 2021 Jul 20;7(1):62. doi: 10.1038/s41531-021-00201-x.
33915983	HDAC6 Signaling at Primary Cilia Promotes Proliferation and Restricts Differentiation of Glioma Cells	Shi P, Hoang-Minh LB, Tian J, Cheng A, Basrai R, Kalaria N, Lebowitz JJ, Khoshbouei H, Deleyrolle LP, Sarkisian MR.	Cancers (Basel). 2021 Apr 1;13(7):1644. doi: 10.3390/cancers13071644.
32275335	Microglia senescence occurs in both substantia nigra and ventral tegmental area	Shaerzadeh F, Phan L, Miller D, Dacquel M, Hachmeister W, Hansen C, Bechtle A, Tu D, Martcheva M, Foster TC, Kumar A, Streit WJ, Khoshbouei H.	Glia. 2020 Nov;68(11):2228-2245. doi: 10.1002/glia.23834. Epub 2020 Apr 10.
27362226	Ki-67 acts as a biological surfactant to disperse mitotic chromosomes	Cuylen S, Blaukopf C, Politi AZ, Müller-Reichert T, Neumann B, Poser I, Ellenberg J, Hyman AA, Gerlich DW.	Nature. 2016 Jul 14;535(7611):308-12. doi: 10.1038/nature18610. Epub 2016 Jun 29.