| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-Alpha-synuclein polyclonal antibody 6438
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to Alpha-synuclein
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-Alpha-synuclein polyclonal antibody 6438
| target relevance |
|---|
| Alpha-synuclein is a crucial protein involved in synaptic function and neurotransmitter release, primarily found in presynaptic terminals of neurons. In research, alpha-synuclein has gained significant attention as a potential cell marker for neurodegenerative diseases, particularly Parkinson's disease. The aggregation of alpha-synuclein into insoluble fibrils is a hallmark of Parkinson's pathology, and its presence in Lewy bodies is a diagnostic feature of the disease. Therefore, alpha-synuclein is utilized as a marker to identify and study Parkinson's disease-related pathology in postmortem brain tissues and experimental models. Antibodies targeting alpha-synuclein are extensively employed in immunohistochemistry and western blotting to visualize and quantify its expression levels in various brain regions and cellular compartments. Click for more on: cell markers and alpha synuclein |
| Protein names Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP) |
| Gene names SNCA,SNCA NACP PARK1 |
| Protein family Synuclein family |
| Mass 14460Da |
| Function Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:28288128, PubMed:30404828, PubMed:20798282, PubMed:26442590). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590). |
| Subellular location Cytoplasm Membrane Nucleus Synapse Secreted Cell projection, axon Note=Membrane-bound in dopaminergic neurons (PubMed:15282274). Expressed and colocalized with SEPTIN4 in dopaminergic axon terminals, especially at the varicosities (By similarity). |
| Tissues Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain. |
| Structure Soluble monomer. Homotetramer (PubMed:21841800). A dynamic intracellular population of tetramers and monomers coexists normally and the tetramer plays an essential role in maintaining homeostasis (PubMed:21841800). Interacts with UCHL1 (By similarity). Interacts with phospholipase D and histones. Interacts (via N-terminus) with synphilin-1/SNCAIP; this interaction promotes formation of SNCA inclusions in the cytoplasm (PubMed:19762560). Interacts with CALM1 (PubMed:23607618). Interacts with STXBP1; this interaction controls SNCA self-replicating aggregation (PubMed:27597756). Interacts with SNARE components VAMP2 and SNAP25; these interactions allows SNARE complex assembly and integrity (PubMed:20798282). Interacts with RPH3A and RAB3A (PubMed:15207266). Interacts with SERF1A; this interaction promotes the aggregation of SNCA (PubMed:22854022, PubMed:31034892). Interacts with SEPTIN4 (By similarity). |
| Post-translational modification Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. ; Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.; Ubiquitinated. The predominant conjugate is the diubiquitinated form. ; Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Target Relevance information above includes information from UniProt accession : P37840 |
| The UniProt Consortium |
Data
 |
| Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, 6438, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, 2539, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. |
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| Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with chicken pAb to alpha-synuclein, 6438, dilution 1:5,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. The alpha-synuclein protein is concentrated in synapses, in particular highlighting the glomeruli synapses in the granule layer and those on Purkinje dendrites in the molecular laye |
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| Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, 6438, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 8 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36543134 | MED13 and glycolysis are conserved modifiers of α-synuclein-associated neurodegeneration | Ren M, Yang Y, Heng KHY, Ng LY, Chong CY, Ng YT, Gorur-Shandilya S, Lee RMQ, Lim KL, Zhang J, Koh TW. | Cell Rep. 2022 Dec 20;41(12):111852. doi: 10.1016/j.celrep.2022.111852. |
| 20648655 | Sensitive and specific detection of α-synuclein in human plasma | Tinsley RB, Kotschet K, Modesto D, Ng H, Wang Y, Nagley P, Shaw G, Horne MK. | J Neurosci Res. 2010 Sep;88(12):2693-700. doi: 10.1002/jnr.22417. |
| 15451225 | Causal relation between alpha-synuclein gene duplication and familial Parkinson's disease | Ibáñez P, Bonnet AM, Débarges B, Lohmann E, Tison F, Pollak P, Agid Y, Dürr A, Brice A. | Lancet. 2004 Sep 25-Oct 1;364(9440):1169-71. doi: 10.1016/S0140-6736(04)17104-3. |
| 15451224 | Alpha-synuclein locus duplication as a cause of familial Parkinson's disease | Chartier-Harlin MC, Kachergus J, Roumier C, Mouroux V, Douay X, Lincoln S, Levecque C, Larvor L, Andrieux J, Hulihan M, Waucquier N, Defebvre L, Amouyel P, Farrer M, Destée A. | Lancet. 2004 Sep 25-Oct 1;364(9440):1167-9. doi: 10.1016/S0140-6736(04)17103-1. |
| 14593171 | alpha-Synuclein locus triplication causes Parkinson's disease | Singleton AB, Farrer M, Johnson J, Singleton A, Hague S, Kachergus J, Hulihan M, Peuralinna T, Dutra A, Nussbaum R, Lincoln S, Crawley A, Hanson M, Maraganore D, Adler C, Cookson MR, Muenter M, Baptista M, Miller D, Blancato J, Hardy J, Gwinn-Hardy K. | Science. 2003 Oct 31;302(5646):841. doi: 10.1126/science.1090278. |
Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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