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Bordetella pertussis Toxin IgG ELISA kit-SERION classic ESR1201G

$364.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
Bordetella pertussis Toxin IgG ELISA Kit
Suitable for IgG detection
Ready-to-use
96 tests

SKU: ESR1201G Category: Virion/Serion kits Tags: Bordetella pertussis, ELISA, IgG

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Bordetella pertussis Toxin reactive IgG
species reactivity	Bordetella pertussis (whooping cough)
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

Bordetella pertussis Toxin IgG ELISA Kit ESR1201G

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgG-, IgA-, IgM-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

target relevance

human antibody anti-Bordetella pertussis

Bordetella pertussis

Description
Bacterium that causes whooping cough (pertussis)

Structure
Bordetella pertussis is a small, Gram-negative, aerobic coccobacillus belonging to the family Alcaligenaceae. The bacterium is a strictly human pathogen and the primary causative agent of pertussis, commonly known as whooping cough. B. pertussis colonizes the ciliated epithelium of the upper respiratory tract through the action of multiple adhesins, including filamentous hemagglutinin, pertactin, and fimbriae. The organism produces several virulence factors, most notably pertussis toxin, adenylate cyclase toxin, and tracheal cytotoxin, which interfere with host immune responses and contribute to respiratory tract damage. Despite widespread vaccination programs, Bordetella pertussis remains a significant cause of respiratory disease worldwide, particularly among infants and inadequately vaccinated populations.

Diagnosis
The diagnosis of Bordetella pertussis infection is performed using microbiological, molecular, and serological methods. Polymerase chain reaction (PCR) testing of nasopharyngeal specimens is the preferred laboratory method during the early stages of infection due to its high sensitivity and rapid turnaround time. Culture of B. pertussis from nasopharyngeal swabs remains the definitive diagnostic method but is less sensitive and requires specialized media and prolonged incubation. Serological assays, including ELISA-based tests that detect antibodies against pertussis toxin and other bacterial antigens, are useful in later stages of disease when bacterial shedding has decreased. Laboratory diagnosis is particularly important because clinical presentation may overlap with other respiratory infections.

Symptoms
Bordetella pertussis infection is highly contagious and spreads through respiratory droplets generated during coughing or sneezing. Following an incubation period of approximately seven to ten days, disease typically progresses through three stages. The catarrhal stage resembles a common upper respiratory infection with symptoms including runny nose, mild fever, sneezing, and occasional cough. This is followed by the paroxysmal stage, characterized by severe repetitive coughing fits that may be followed by the characteristic inspiratory 'whoop,' post-tussive vomiting, and exhaustion. In infants, apnea may occur and can be life-threatening. The final convalescent stage involves gradual recovery over weeks to months, although coughing episodes may persist. Complications include pneumonia, seizures, encephalopathy, rib fractures, and, particularly in young infants, death.

Data

FAQ & Publications

Frequently Asked Questions

What is the target antigen detected by the Bordetella pertussis Toxin IgG ELISA Kit ESR1201G?

The kit targets the Bordetella pertussis Toxin reactive IgG antibodies, which are specific to the pertussis toxin produced by Bordetella pertussis.

Which sample types are compatible with the Bordetella pertussis Toxin IgG ELISA Kit ESR1201G?

This ELISA kit is suitable for use with serum, plasma, and whole blood samples.

How should the Bordetella pertussis Toxin IgG ELISA Kit ESR1201G be stored to maintain stability?

The kit components should be stored at a temperature between 2 and 8 degrees Celsius to ensure stability and optimal performance.

What is the assay format and principle used in the Bordetella pertussis Toxin IgG ELISA Kit ESR1201G?

The kit utilizes an indirect and quantitative ELISA assay format for detection of IgG antibodies against Bordetella pertussis toxin.

How many tests are provided in one kit of the Bordetella pertussis Toxin IgG ELISA Kit ESR1201G?

Each kit contains ready-to-use components sufficient to perform 96 tests using break-apart microtiter strips.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR1201G protocol

Documents

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Bordetella pertussis Toxin IgG ELISA kit-SERION classic ESR1201G

Data

FAQ & Publications

Protocols

Documents

Related Technical Resources & Posts

Reviews

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