Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | chicken |
isotype | IgY |
clonality | polyclonal |
concentration | 1 mg/mL |
applications | ELISA |
available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-DYDDDDK tag polyclonal antibody 2933
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to DYDDDDK tag
- Suitable for: ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-DYDDDDK tag polyclonal antibody 2933
antibody |
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Database link: DYDDDDK tag (FLAG tag) |
Tested applications ELISA |
Recommended dilutions user optimized |
Immunogen DYKDDDDK (FLAG) synthetic peptide conjugated to KLH |
Size and concentration 25, 100, 1000µg and 1 mg/mL |
Form liquid |
Storage Instructions 2-8°C |
Storage buffer PBS, pH 7.2, 0.09% NaN3 |
Purity affinity purified |
Clonality polyclonal |
Isotype IgY |
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723 |
Isotype control Chicken polyclonal - Isotype Control |
target relevance |
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DYDDDDK (Flag) tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify DYDDDDK (Flag) tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, DYDDDDK (Flag) tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and DYDDDDK (Flag) tag |
Biotechnology The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples. The versatility and efficiency of the DYDDDDK tag have revolutionized the field of molecular biology, aiding researchers in studying protein-protein interactions, subcellular localization, and protein dynamics, ultimately advancing our understanding of cellular processes and driving innovative discoveries in various scientific disciplines. |
Data
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.pmid | title | authors | citation |
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Protocols
relevant to this product |
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ELISA |
Documents
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