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HEK 293 HCPs (HEK 293 host cell proteins) ELISA Kit 60801

$570.00

Summary

  • ELISA kit for research use (RUO)
  • Reactivity: Hek293 proteins
  • Suitable for: ELISA
  • Time:3 hours
  • Sample types: Serum, plasma, tissue homogenates and other biological fluids
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

HEK 293 HCPs (HEK 293 host cell proteins)

species reactivity

human

applications

sandwich (sELISA)

assay type

direct & quantitative

available size

96 tests

sensitivity

46.875 ng/ml

range

78.125–5000 ng/ml

HEK 293 HCPs (HEK 293 host cell proteins) ELISA Kit

kit
Assay type
sandwich (sELISA)
Research area
host cell protein
Sample type
Serum, plasma, tissue homogenates and other biological fluids
Components
Break apart microtiter test strips each coated single wells 8 x 12 (96 Total)
Lyophilized Standard2 x vial
Biotin-labeled Antibody(Concentrated, 100X)120 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)120 uL
Washing solution concentrate (25X)30 mL
Sample Dilution buffer20 mL
Antibody Dilution buffer10 mL
Streptavidin Dilution buffer10 mL
Stopping solution10 mL
TMB Substrate (ready-to-use)10 mL
Plate seals3
Storage
Store at 2-8°C.
target relevance
HEK293 cell proteins
Protein names
HEK293 host cell proteins
Alternative names
HEK293 HCP, Human embryonic kidney host cell proteins, 293 cell proteins
Description
Residual proteins derived from HEK293 cells used for recombinant protein, viral vector, and gene therapy manufacturing.

Data

Standard Curve

X axis: log | Y axis: linear
Standard STD [ng/mL] OD1 OD2 Average OD Corrected OD
Blank #8 0 0.12 0.12 0.12 0
Standard #7 78.13 0.18 0.19 0.19 0.07
Standard #6 156.25 0.24 0.25 0.25 0.13
Standard #5 312.5 0.36 0.38 0.37 0.25
Standard #4 625 0.56 0.59 0.57 0.45
Standard #3 1250 0.9 0.95 0.92 0.8
Standard #2 2500 1.37 1.44 1.41 1.29
Standard #1 5000 2.27 2.39 2.33 2.21

Precision

Sample n Mean Standard Deviation CV (%)
Intra Sample 1 20 159.8 7.97 4.99
Inter Sample 1 20 151.8 7.09 4.67
Intra Sample 2 20 593.7 28.32 4.77
Inter Sample 2 20 616.7 27.07 4.39
Intra Sample 3 20 2417 113.36 4.69
Inter Sample 3 20 2436 111.81 4.59

Stability

37°C 1 Month (%) 2–8°C 6 Months (%) 2–8°C 12 Months (%)
80 95-100 85-98

FAQ & Publications

Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

Relevant to this product
View Protocol  |  View PDF

Documents

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