| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2a |
| clonality | monoclonal |
| concentration | concentrate, predilute |
| applications | IHC |
| reactivity | human |
| available size | 0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted |
mouse anti-Myelin Basic Protein (MBP) monoclonal antibody ZM202 6274
$160.00 – $528.00
Antibody summary
- Mouse monoclonal to Myelin Basic Protein (MBP)
- Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
- Reacts with: Human
- Isotype:IgG2a
- Control: Brain
- Visualization: Cytoplasmic
- 0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted
mouse anti-Myelin Basic Protein (MBP) monoclonal antibody ZM202 6274
| target relevance |
|---|
| Protein names Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein) |
| Protein family Myelin basic protein family |
| Mass 33117Da |
| Function The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. |
| Subellular location Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.; [Isoform 3]: Nucleus . Note=Targeted to nucleus in oligodendrocytes. |
| Tissues MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. |
| Structure Homodimer. Isoform 3 exists as a homodimer. |
| Post-translational modification PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.; PTM: The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).; PTM: Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.; PTM: Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells.; PTM: Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. |
| Involvement in disease Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). |
| Target Relevance information above includes information from UniProt accession: P02686 |
| The UniProt Consortium |
|
| Human brain white mater stained with anti-MBP antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of glial cells. |
Publications
| pmid | title | authors | citation |
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| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| IHC |
| # | SDS | Certificate | |
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| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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