| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
rabbit anti-HA tag polyclonal antibody 6150
$426.00
Antibody summary
- Rabbit polyclonal to HA tag
- Suitable for: WB, ICC/IF, IP, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg
rabbit anti-HA tag polyclonal antibody 6150
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are |
| Immunogen HA (YPYDVPDYA) conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| HA tag |
| Protein names Hemagglutinin epitope tag |
| Alternative names HA tag, HA epitope, Influenza HA tag, YPYDVPDYA tag |
| Function The HA tag is an artificial peptide epitope commonly fused to recombinant proteins to facilitate detection, purification, localization, and immunoprecipitation using anti-HA antibodies. |
| Description The HA tag is a synthetic peptide epitope derived from the human influenza A virus hemagglutinin (HA) protein. It is widely used for detection, purification, and characterization of recombinant proteins. |
| Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein |
| Sequence YPYDVPDYA |
| Structure The HA tag is a 9-amino acid peptide epitope with the sequence YPYDVPDYA derived from the influenza A virus hemagglutinin protein. |
| Function The HA tag is used as a molecular tag for detection, purification, immunoprecipitation, and localization of recombinant proteins. |
| Detection HA-tagged proteins are commonly detected using anti-HA monoclonal antibodies such as clones 12CA5, 3F10, HA.11, and C29F4. |
| Applications HA-tagged proteins are routinely analyzed by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation, and affinity purification. |
| Research relevance The HA tag is one of the most widely used epitope tags in molecular biology and biotechnology and is often used alongside FLAG, Myc, V5, and His tags. |
Data
 |
| Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified rabbit anti-HA antibody 6150 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified rabbit anti-HA antibody 6150 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-HA tag polyclonal antibody 6150 validated for?
This antibody is validated for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and ELISA.
How should the rabbit anti-HA tag polyclonal antibody 6150 be stored to maintain stability?
The antibody should be stored at 2-8°C for at least one year to maintain stability.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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