Skip to content

rabbit anti-p53DINP1 polyclonal antibody 5900

$445.00

Antibody summary

  • Rabbit polyclonal to p53DINP1
  • Suitable for: ELISA,WB,IHC-P,IF
  • Isotype: Whole IgG
  • 100 µg
SKU: 5900parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

p53DINP1

available sizes

100 µg

rabbit anti-p53DINP1 polyclonal antibody 5900

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 0.5-1ug/mL

IHC: use at 2ug/mL. Detection of p53DINP1 in mouse liver with #56223 at 2ug/mL.

Immunofluorescence: use at 5-20ugml. Detection of p53DINP1 in human lung tissue lysate with #56223 at (A) 0.5ug/mL and (B) 1ug/mL. Detection of p53DINP1 in human liver w
Immunogen
Peptide corresponding to aa 1-14 of human p53DINP1.
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4, 0.02% NaN3.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Tumor protein p53-inducible nuclear protein 1 (Stress-induced protein) (p53-dependent damage-inducible nuclear protein 1) (p53DINP1)
Gene names
TP53INP1,TP53INP1 P53DINP1 SIP
Mass
27366Da
Function
Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC.
Subellular location
Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Note=Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.
Tissues
Ubiquitously expressed.
Structure
Interacts with p53/TP53 and HIPK2. Interacts with PRKCG, GABARAP, GABARAPL1, GABARAPL2, MAP1LC3A, MAP1LC3B and MAP1LC3C.
Target Relevance information above includes information from UniProt accession: Q96A56
The UniProt Consortium

Data

benchmark-antibodies_anti-p53dinp1_antibody_5900_1.jpg
Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 µg/mL.
benchmark-antibodies_anti-p53dinp1_antibody_5900_2.jpg
Immunohistochemical staining of mouse liver using p53DINP1 antibody at 2 µg/mL.
benchmark-antibodies_anti-p53dinp1_antibody_5900_3.gif
Immunofluorescence of p53DINP1 in human liver tissue with p53DINP1 antibody at 5 µg/mL. Green: p53DINP1 antibody (5900)
Red: Phylloidin staining
Blue: DAPI staining

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

#
Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information.

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.