| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | IRAK-M |
| available sizes | 100 µg |
rabbit anti-IRAK-M BP polyclonal antibody 2359
$445.00
Antibody summary
- Rabbit polyclonal to IRAK-M BP
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-IRAK-M BP polyclonal antibody 2359
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: Tissue lysate from mouse spleen or rat kidney. |
| Immunogen Peptide corresponding to aa 581-596 of human IRAK-M. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Interleukin-1 receptor-associated kinase 3 (IRAK-3) (IL-1 receptor-associated kinase M) (IRAK-M) (Inactive IL-1 receptor-associated kinase 3) |
| Gene names IRAK3,IRAK3 |
| Protein family Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily |
| Mass 67767Da |
| Function FUNCTION: Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:10383454, PubMed:29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383). {ECO:0000250|UniProtKB:Q8K4B2, ECO:0000269|PubMed:10383454, ECO:0000269|PubMed:29686383}. |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29686383}. Nucleus {ECO:0000269|PubMed:29686383}. Note=In dendritic cells, translocates into the nucleus upon IL33 stimulation. {ECO:0000250|UniProtKB:Q8K4B2}. |
| Tissues TISSUE SPECIFICITY: Expressed in eosinophils, dendritic cells and/or monocytes (at protein level) (PubMed:29686383). Expressed predominantly in peripheral blood lymphocytes (PubMed:10383454). {ECO:0000269|PubMed:10383454, ECO:0000269|PubMed:29686383}. |
| Structure SUBUNIT: Monomer (PubMed:33238146). Homodimer; disulfide-linked (PubMed:33238146). May interact with IRAK4 (when phosphorylated) (PubMed:33238146). Interacts (when phosphorylated at Ser-110) with PIN1 (via WW domain) in response to IL33-mediated (but not TLR4 ligand LPS) dendritic cell stimulation (PubMed:29686383). {ECO:0000269|PubMed:29686383, ECO:0000269|PubMed:33238146}. |
| Domain DOMAIN: The nucleotide binding domain binds ATP with low affinity. {ECO:0000269|PubMed:33238146}. |
| Involvement in disease DISEASE: Asthma-related traits 5 (ASRT5) [MIM:611064]: Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy and atopic dermatitis. {ECO:0000269|PubMed:17503328}. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: Q9Y616 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of IRAK-M in (M) mouse spleen and (R) rat liver tissue lysates with IRAK-M antibody at 1 µg/mL. |
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| Immunohistochemistry of IRAK-M in human spleen tissue with IRAK-M antibody at 5 µg/mL. |
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| Immunofluorescence of IRAK-M in Rat Liver tissue with IRAK-M antibody at 10 µg/mL. |
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| Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/mL. |
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| Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 µg/mL.
Red: IRAK-M Antibody (2359) Blue: DAPI staining |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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