| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Bnip3L (IN) |
| available sizes | 100 µg |
rabbit anti-Bnip3L (IN) polyclonal antibody 5958
$445.00
Antibody summary
- Rabbit polyclonal to Bnip3L (IN)
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-Bnip3L (IN) polyclonal antibody 5958
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate of human K562 cells. |
| Immunogen Peptide corresponding to aa 77- 92 of human Bnip3L (accession no. AF067396) This amino acid sequence is identical to that of mouse. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (Adenovirus E1B19K-binding protein B5) (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A) (NIP3-like protein X) (NIP3L) |
| Gene names BNIP3L,BNIP3L BNIP3A BNIP3H NIX |
| Protein family NIP3 family |
| Mass 23930Da |
| Function FUNCTION: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. {ECO:0000269|PubMed:10381623, ECO:0000269|PubMed:21264228}. |
| Subellular location SUBCELLULAR LOCATION: Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. Note=Colocalizes with SPATA18 at the mitochondrion outer membrane. |
| Structure SUBUNIT: Self-associates. Interacts with BNIP3 and STEAP3. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains). Interacts with PPTC7; this interaction promotes BNIP3L degradation (PubMed:38992176). {ECO:0000269|PubMed:10381623, ECO:0000269|PubMed:12606722, ECO:0000269|PubMed:21264228, ECO:0000269|PubMed:38992176}.; SUBUNIT: (Microbial infection) Interacts with human adenovirus-2 E1B 19 kDa protein. {ECO:0000269|PubMed:10381623}.; SUBUNIT: (Microbial infection) Interacts with Kaposi's sarcoma-associated herpesvirus protein VIRF-1. {ECO:0000269|PubMed:31324791}. |
| Post-translational modification PTM: Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. |
| Target Relevance information above includes information from UniProt accession: O60238 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of Bnip3L in K562 whole cell lysate in (A) the absence, or (B) presence of immunogenic peptide with Bnip3L antibody at 1 µg/mL. |
 |
| Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 µg/mL. |
 |
| Immunofluorescence of Bnip3L in Human Kidney tissue with Bnip3L antibody at 10 µg/mL. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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