| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Interleukin-21 (IL-21) (IN) |
| available sizes | 100 µg |
rabbit anti-IL-21 (IN) polyclonal antibody 6228
$469.00
Antibody summary
- Rabbit polyclonal to IL-21 (IN)
- Suitable for: ELISA,WB,ICC
- Isotype: Whole IgG
- 100 µg
rabbit anti-IL-21 (IN) polyclonal antibody 6228
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 18 kD is detected. Positive control: HL60 or THP-1 cell lysates. |
| Immunogen Peptide corresponding to aa 121-135 of human IL-21 precursor. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Interleukin-21 (IL-21) (Za11) |
| Gene names IL21,IL21 |
| Protein family IL-15/IL-21 family |
| Mass 18653Da |
| Function FUNCTION: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (PubMed:11081504, PubMed:15178704). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity). {ECO:0000250|UniProtKB:Q9ES17, ECO:0000269|PubMed:11081504, ECO:0000269|PubMed:15178704}. |
| Subellular location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11081504}. |
| Tissues TISSUE SPECIFICITY: Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. {ECO:0000269|PubMed:11081504}. |
| Involvement in disease DISEASE: Immunodeficiency, common variable, 11 (CVID11) [MIM:615767]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. {ECO:0000269|PubMed:24746753}. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: Q9HBE4 |
| The UniProt Consortium |
Data
 |
| Western Blot Validation in Human, Mouse and Rat Cell Lines Loading: 15 µg of lysates per lane. Antibodies: IL-21 6228, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
 |
| Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 µg of lysates per lane. Antibodies: IL-21 6228 (1 µg/mL), IL-21 2463 (5 µg/mL), beta-actin (5 µg/mL) and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
 |
| Western Blot Validation in Human HL-60 Cell Lysate Loading: 15 µg of lysates per lane. Antibodies: IL-21 6228 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide. |
 |
| Immunocytochemistry Validation of IL-21 in K562 Cells Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (6228) at 2 µg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
 |
| Induced Expression Validation of IL-21 expresssion in patients with Crohn'sDisease (Monteleone et al, 2416) Enhanced IL-21 was observed in involved but not uninvolvedCD, and was not associated with any CD phenotype, such as fibrostenosing disease. IL-21 expression was detected by anti-IL-21 antibodies (6228). |
 |
| Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD) (Sarra et al., 2412) (b) shows IL-21 expression levels treated with dimethyl sulfoxide (DMSO) or wortmannin (WRT). (c) shows IL-21 expression levels with the inhibition of IL-15 antibody (aIL-15). IgG isotype was used as a control.Both WB show IL-21 expression decreased with the treatment with WRT or the inhibition of IL-15 antibody. |
 |
| Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp) (Caruso et al., 2416) (A) shows IL-21 expression levels from biopsies of patients with Hp infection and without Hp infection (n=3).(C) shows IL-21 expression levels in CD3+LPMC cells from Hp-positive and Hp-negative patients (n=3).Both WB show IL-21 expression increased in patients with Hp infection. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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