| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | HA |
| available sizes | 100 µg |
rabbit anti-HA HRP polyclonal antibody 2583
$513.00
Antibody summary
- Rabbit polyclonal to HA , HRP conjugate
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
rabbit anti-HA HRP polyclonal antibody 2583
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. |
| Immunogen HA (YPYDVPDYA) conjugated to KLH. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| HA tag |
| Protein names Hemagglutinin epitope tag |
| Alternative names HA tag, HA epitope, Influenza HA tag, YPYDVPDYA tag |
| Function The HA tag is an artificial peptide epitope commonly fused to recombinant proteins to facilitate detection, purification, localization, and immunoprecipitation using anti-HA antibodies. |
| Description The HA tag is a synthetic peptide epitope derived from the human influenza A virus hemagglutinin (HA) protein. It is widely used for detection, purification, and characterization of recombinant proteins. |
| Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein |
| Sequence YPYDVPDYA |
| Structure The HA tag is a 9-amino acid peptide epitope with the sequence YPYDVPDYA derived from the influenza A virus hemagglutinin protein. |
| Function The HA tag is used as a molecular tag for detection, purification, immunoprecipitation, and localization of recombinant proteins. |
| Detection HA-tagged proteins are commonly detected using anti-HA monoclonal antibodies such as clones 12CA5, 3F10, HA.11, and C29F4. |
| Applications HA-tagged proteins are routinely analyzed by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation, and affinity purification. |
| Research relevance The HA tag is one of the most widely used epitope tags in molecular biology and biotechnology and is often used alongside FLAG, Myc, V5, and His tags. |
Data
 |
| Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-HA antibody 2583 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications is the Rabbit anti-HA HRP Polyclonal Antibody 2583 validated for?
This antibody has been tested and is suitable for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA.
How should I store the Rabbit anti-HA HRP Polyclonal Antibody 2583 to maintain its stability?
The antibody is stable for at least one year when stored at 2-8°C in its supplied form.
What is the recommended dilution range for using this antibody in Western blot applications?
For immunoblotting, the recommended dilution range is from 1:1,000 to 1:30,000; however, users should optimize dilution conditions for their specific experiments.
What is the immunogen used to generate the Rabbit anti-HA HRP Polyclonal Antibody 2583?
The immunogen is the HA peptide sequence YPYDVPDYA conjugated to keyhole limpet hemocyanin (KLH).
Which species does this antibody target and what is its clonality and isotype?
This antibody is a rabbit polyclonal antibody of the IgG isotype that targets the HA tag, a hemagglutinin epitope sequence.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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