SARS-CoV-2 IgG ELISA Kit MD-7304

SARS-CoV-2 IgG ELISA Kit MD-7304

kit
Assay type Indirect ELISA
Research area Infectious Disease
Sample type Serum, plasma, whole blood
Components Each set of reagents contains: Wash buffer (10x concentrate) Contains phosphate buffer, NaCl and detergent. Preservatives: MIT (0.01%) and Oxypyrion (0.1%) 100 mL Dilution buffer (ready-to-use) Contains protein, detergent and blue dye. Preservatives: MIT (0.01%) and Oxypyrion (0.1%) 125 mL Chromogenic substrate tetramethylbenzidine (TMB, ready-to-use) 12 mL Stop solution 24.9% phosphoric acid (H?PO?) 12 mL Instructions for use 1 Evaluation sheet 1 Sealing film 2 sheets recomWell SARS-CoV-2 IgG also contains: 12 x 8 wells microtitre plate coated with recombinant SARS-CoV-2 antigen in a vacuum-sealed pouch 1 Positive control (purple cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µL Cut-off control (yellow cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µL Negative control (white cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µL Anti-human IgG conjugate (101x concentrate, red cap) Contains NaN? (<0.1%), MIT (<0.01%) and chloracetamide (<0.1%) 500 µL recomWell SARS-CoV-2 IgA also contains: 12 x 8 wells microtitre plate (strip marked blue) coated with recombinant SARS-CoV-2 antigen in a vacuum-sealed pouch 1 Positive control (brown cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µL Cut-off control (orange cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µL Negative control (white cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µL Anti-human IgA conjugate (101x concentrate, blue cap) Contains NaN? (<0.1%), MIT (<0.01%) and chloracetamide (<0.1%) 500 µL
Storage Store at 2-8°C.

target relevance
Organism SARS-CoV-2
Structure and strains SARS-CoV-2 consists of 4 structural proteins and 16 non-structural proteins. The 4 structural proteins are spike (S), envelope (E), nucleocapsid (N) and membrane (M) protein. The N protein is associated with the RNA genome, the S, E and M proteins together form the virus envelope. The S protein is a glycoprotein consisting of two domains, the S1 domain, which contains the receptor binding domain (RBD) responsible for the virus being able to attach to and fuse with the ACE2 receptor of the host cell, and the S2 domain including transmembrane and endodomain. On the virus surface, the S protein forms trimeric structures. N and S protein are major immunogenic proteins of the virus family Coronaviridae and are very well suited for serological detection of anti-SARS-CoV-2 antibodies. Recombinant versions of both proteins are already widely used in different test systems.
Disease Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a strain of coronavirus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. The virus previously had the provisional name 2019 novel coronavirus (2019-nCoV),and has also been called human coronavirus 2019 (HCoV-19 or hCoV-19). SARS-CoV-2 is a positive-sense single-stranded RNA virus that is contagious in humans.
Detection and diagnosis Neutralizing antibodies during host immune responses are predominantly directed against S protein, which is supposedly suitable for very specific test settings as it is less conserved within the family of Coronaviridae than the other immunogenic antigens. However, recent studies have shown that antibodies against N protein are detectable earlier in mild infections than antibodies directed against S protein. As an acute marker in particular, it can be beneficial to use N protein for most sensitive early detection. In the further course of the immune response, the N protein antibody level decreases, whereas antibodies against S protein are detectable for a longer time. Various studies show that anti-SARS-CoV-2 antibodies of the IgA and IgM class are detectable in the median at the earliest from about six days after the onset of symptoms, increase in the further course of the disease and decrease again between 18 and 35 days. Antibodies of the IgG class are detectable in the median ten to eighteen days after onset of symptoms and are detectable over several months.