Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human stomach stained with anti MUC-5AC antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of gastric body glands

rabbit anti-MUC-5AC monoclonal antibody (ZR19) 6269

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to MUC-5AC
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Gastric carcinoma
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6269parent Category: antibody Tags: anatomy, GI Pathology, monoclonal, MUC-5AC

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-MUC-5AC monoclonal antibody ZR19 6269

antibody
Database link: human P98088
Tested applications IHC
Recommended dilutions As directed
Immunogen Recombinant full-length human MUC5AC protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Mucin-5AC (MUC-5AC) (Gastric mucin) (Major airway glycoprotein) (Mucin-5 subtype AC, tracheobronchial) (Tracheobronchial mucin) (TBM)
Mass 585570Da
Function Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
Subellular location Secreted .
Tissues Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
Structure Homomultimer; disulfide-linked (PubMed:14718370). The N- and C-terminus mediate their assembly into higher order structures to form filaments (By similarity). The CTCK domains of two polypeptides associate in the endoplasmic reticulum to generate intermolecularly disulfide-bonded dimers (By similarity). These dimers progress to the Golgi apparatus, which is a more acidic environment than the endoplasmic reticulum. Under acidic conditions, the N-termini form non-covalent intermolecular interactions that juxtapose assemblies from different CTCK-linked dimers to produce long, disulfide-linked polymers that remain highly compact until secretion (By similarity).
Post-translational modification PTM: C-, O- and N-glycosylated (PubMed:14718370). O-glycosylated on the second and last Thr of the Thr-/Ser-rich tandem repeats TTPSPVPTTSTTSA (PubMed:14718370, PubMed:22186971, PubMed:25939779). One form of glycosylation is also known as Lewis B (LeB) blood group antigen, a tetrasaccharide consisting of N-acetylglucosamine having a fucosyl residue attached (PubMed:14535999). It has a role as an epitope and antigen and functions as a receptor for H.pylori binding and facilitates infection (PubMed:14535999). C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis (PubMed:14718370).; PTM: Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide.
Domain DOMAIN 79..249; /note="VWFD 1"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU00580"; DOMAIN 338..394; /
Target Relevance information above includes information from UniProt accession: P98088
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human stomach stained with anti MUC-5AC antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of gastric body glands

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.