mouse anti-TRIP230 monoclonal antibody (10G5) 4407

$503.00

Antibody summary

Mouse monoclonal to TRIP230
Suitable for: WB,ICC/IF,IP
Isotype: IgG1
100 µg

SKU: 4407parent Category: antibody Tags: monoclonal, mouse, TRIP230

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	TRIP230
available sizes	100 µg

mouse anti-TRIP230 monoclonal antibody (10G5) 4407

antibody

Tested applications WB,ICC/IF,IP
Recommended dilutions Immunoblotting, Immunoprecipitation: use at 1-5 ug/mL. Positive control: MCF7, HBL100, and T24 cells and recombinant fusion protein.
Immunogen GST fusion protein corresponding to aa 1099-1372 of Trip230 expressed in E. coli.
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Storage buffer PBS, pH 7.4
Purity protein affinty purification
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Protein names Thyroid receptor-interacting protein 11 (TR-interacting protein 11) (TRIP-11) (Clonal evolution-related gene on chromosome 14 protein) (Golgi-associated microtubule-binding protein 210) (GMAP-210) (Trip230)
Gene names TRIP11,TRIP11 CEV14
Mass 227586Da
Function FUNCTION: Is a membrane tether required for vesicle tethering to Golgi. Has an essential role in the maintenance of Golgi structure and function (PubMed:25473115, PubMed:30728324). It is required for efficient anterograde and retrograde trafficking in the early secretory pathway, functioning at both the ER-to-Golgi intermediate compartment (ERGIC) and Golgi complex (PubMed:25717001). Binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. {ECO:0000269\|PubMed:10189370, ECO:0000269\|PubMed:25473115, ECO:0000269\|PubMed:25717001, ECO:0000269\|PubMed:30728324, ECO:0000269\|PubMed:9256431}.
Subellular location SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane {ECO:0000269\|PubMed:10189370, ECO:0000269\|PubMed:25473115}; Peripheral membrane protein {ECO:0000269\|PubMed:10189370}. Cytoplasm, cytoskeleton {ECO:0000269\|PubMed:10189370}. Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269\|PubMed:25473115}. Note=Associates with the ends of centrosome-nucleated microtubules.
Tissues TISSUE SPECIFICITY: Highly expressed in pancreas, muscle, heart, testis, peripheral blood leukocytes, and in several leukemia cell lines. Detected at intermediate levels in placenta and kidney, and at low levels in brain and lung. Isoform 1 and isoform 2 are expressed in articular chondrocytes (PubMed:30728324). {ECO:0000269\|PubMed:10189370, ECO:0000269\|PubMed:30728324, ECO:0000269\|PubMed:9373237}.
Structure SUBUNIT: Interacts with the active form of RAB2A (PubMed:25473115). Interacts with IFT20 (PubMed:19112494). Binds RB1. {ECO:0000269\|PubMed:19112494, ECO:0000269\|PubMed:25473115}.
Target Relevance information above includes information from UniProt accession : Q15643
The UniProt Consortium

Data

Immunofluorescence analysis of methanol-fixed HeLa, using TRIP230(4407) antibody at 1:100 dilution.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.