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mouse anti-Transglutaminase-3 (TG3) monoclonal antibody (C2D) 7739

$520.00

Antibody summary

  • Mouse monoclonal to Transglutaminase-3 (TG3)
  • Suitable for: WB,ICC,IHC
  • Isotype: IgG1
  • 100 µg
SKU: 7739parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Transglutaminase-3 (TG3)

available sizes

100 µg

Available product – mouse anti-Transglutaminase-3 (TG3) monoclonal antibody (C2D) 7739

antibody
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
Immunoblotting: use at 1-5ug/mL. Bands of ~77kDa and ~30kDa are detected.

Immunocytochemistry: use at 2-10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Recombinant human TG3 (accession no. Q08188).
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Storage buffer
Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Protein-glutamine gamma-glutamyltransferase E (EC 2.3.2.13) (Transglutaminase E) (TG(E)) (TGE) (TGase E) (Transglutaminase-3) (TGase-3) [Cleaved into: Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chain; Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain]
Gene names
TGM3,TGM3
Protein family
Transglutaminase superfamily, Transglutaminase family
Mass
76632Da
Function
FUNCTION: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. {ECO:0000250}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+); Xref=Rhea:RHEA:54816, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:10207, Rhea:RHEA-COMP:14005, ChEBI:CHEBI:28938, ChEBI:CHEBI:29969, ChEBI:CHEBI:30011, ChEBI:CHEBI:138370; EC=2.3.2.13; Evidence={ECO:0000255|PROSITE-ProRule:PRU10024, ECO:0000269|PubMed:11980702, ECO:0000269|PubMed:12679341, ECO:0000269|PubMed:27866708};
Subellular location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27866708}.
Structure
SUBUNIT: Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide. {ECO:0000269|PubMed:11980702, ECO:0000269|PubMed:12679341}.
Post-translational modification
PTM: Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075). {ECO:0000269|PubMed:16565075, ECO:0000269|PubMed:8099584}.
Target Relevance information above includes information from UniProt accession : Q08188
The UniProt Consortium

Data

benchmark-antibodies_anti-transglutaminase-3_tg3_antibody_7739_1.jpg
Immunoblotting: use at 1-5ug/ml. Bands of ~77kDa and ~30kDa are detected. Detection of full-length and proteolysed TG3 in lysate of human epidermal cells with #7739.
benchmark-antibodies_anti-transglutaminase-3_tg3_antibody_7739_2.jpg
Immunocytochemistry: use at 2-10ug/ml. Detection of TG3 in human foreskin with #7739.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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