Name: mouse anti-SRC-1 monoclonal antibody (H4) 5818
SKU: 5818parent
Price: 503.00 USD
Availability: InStock

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	SRC-1
available sizes	100 µg

mouse anti-SRC-1 monoclonal antibody (H4) 5818

antibody

Tested applications WB,IP,ChIP
Recommended dilutions Immunoblotting and Immunoprecipitation: use at 0.5-2 ug/mL. Positive control: COS cells
Immunogen Human SRC-1 (aa 477-947) gst-fusion protein.
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Storage buffer 10 mM PBS, pH 7.4.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Protein names Nuclear receptor coactivator 1 (NCoA-1) (EC 2.3.1.48) (Class E basic helix-loop-helix protein 74) (bHLHe74) (Protein Hin-2) (RIP160) (Renal carcinoma antigen NY-REN-52) (Steroid receptor coactivator 1) (SRC-1)
Gene names NCOA1,NCOA1 BHLHE74 SRC1
Protein family SRC/p160 nuclear receptor coactivator family
Mass 156757Da
Function FUNCTION: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. {ECO:0000269\|PubMed:10449719, ECO:0000269\|PubMed:12954634, ECO:0000269\|PubMed:7481822, ECO:0000269\|PubMed:9223281, ECO:0000269\|PubMed:9223431, ECO:0000269\|PubMed:9296499, ECO:0000269\|PubMed:9427757}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=acetyl-CoA + L-lysyl-[protein] = CoA + H(+) + N(6)-acetyl-L-lysyl-[protein]; Xref=Rhea:RHEA:45948, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:10731, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:61930; EC=2.3.1.48;
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00981}.
Tissues TISSUE SPECIFICITY: Widely expressed. {ECO:0000269\|PubMed:15313887, ECO:0000269\|PubMed:9427757}.
Structure SUBUNIT: Interacts with PPARA; the interaction is direct (PubMed:11698662). Interacts with PPARG; the interaction is direct (PubMed:11698662, PubMed:9744270). Interacts with ESRRG; the interaction is direct (PubMed:11864604). Interacts with STAT5A (via FDL motif) (PubMed:12954634). Interacts with STAT5B (via FDL motif) (PubMed:12954634). Interacts with STAT6 (via LXXLL motif) (PubMed:12138096). Interacts (via LXXLL 1, 2 and 3 motifs) with RORC (via AF-2 motif) (By similarity). Interacts with ASXL1 (PubMed:16606617). Interacts with the methyltransferase CARM1 (By similarity). Interacts with COPS5 (PubMed:10722692). Interacts with the histone acetyltransferase CREBBP (By similarity). Interacts with DDX5 (PubMed:11250900). Interacts with the histone acetyltransferase EP300 (By similarity). Interacts with ESR1 (PubMed:7481822). Interacts with GCCR (PubMed:7481822). Interacts with the basal transcription factor GTF2B (PubMed:8754792). Interacts with NCOA6 (PubMed:10567404). Interacts with NCOA2 (PubMed:10594042). Interacts with NR3C1 (PubMed:12917342). Interacts with NR4A1/Nur77 (PubMed:18690216). Interacts with NR4A3 (By similarity). Interacts with PCAF (PubMed:9296499). Interacts with PGR (PubMed:7481822). Interacts with PRMT2 (PubMed:12039952). Interacts with PRMT6 (PubMed:20047962). Interacts with PSMB9 (PubMed:16957778). Interacts with RXRA, the interaction is ligand-dependent (PubMed:19786558, PubMed:7481822). Interacts with STAT3 following IL-6 stimulation (PubMed:11773079). Interacts with TRA (PubMed:7481822). Interacts with TRIP4 (PubMed:25219498). Interacts with TTLL5/STAMP (PubMed:17116691). Interacts with UBE2L3; they functionally interact to regulate progesterone receptor transcriptional activity (PubMed:15367689). Interacts with VDR (PubMed:28698609). {ECO:0000250\|UniProtKB:P70365, ECO:0000269\|PubMed:10567404, ECO:0000269\|PubMed:10594042, ECO:0000269\|PubMed:10722692, ECO:0000269\|PubMed:11250900, ECO:0000269\|PubMed:11698662, ECO:0000269\|PubMed:11773079, ECO:0000269\|PubMed:11864604, ECO:0000269\|PubMed:12039952, ECO:0000269\|PubMed:12138096, ECO:0000269\|PubMed:12917342, ECO:0000269\|PubMed:12954634, ECO:0000269\|PubMed:15367689, ECO:0000269\|PubMed:16606617, ECO:0000269\|PubMed:16957778, ECO:0000269\|PubMed:17116691, ECO:0000269\|PubMed:18690216, ECO:0000269\|PubMed:19786558, ECO:0000269\|PubMed:20047962, ECO:0000269\|PubMed:25219498, ECO:0000269\|PubMed:28698609, ECO:0000269\|PubMed:7481822, ECO:0000269\|PubMed:8754792, ECO:0000269\|PubMed:9296499, ECO:0000269\|PubMed:9744270}.
Post-translational modification PTM: Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein. {ECO:0000269\|PubMed:12529333}.; PTM: Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites. {ECO:0000269\|PubMed:12529333}.
Target Relevance information above includes information from UniProt accession : Q15788
The UniProt Consortium

Data

Mouse tissue (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] (5818) diluted at 1:2000.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmid	title	authors	citation

Protocols

relevant to this product
Western blot

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