mouse anti-S-100 monoclonal antibody (4B3) 5838

$338.00

Antibody summary

Mouse monoclonal to S-100
Suitable for: WB,ELISA,AP
Isotype: IgG2a
100 µg

SKU: 5838parent Category: antibody Tags: monoclonal, mouse, S-100

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	S100α/β
available sizes	100 µg

mouse anti-S-100 monoclonal antibody (4B3) 5838

antibody

Tested applications WB,ELISA
Recommended dilutions These antibodies may be used in ELISA and Western blot (human brain): 56203, 56204, and 56206 may be used to affinity-purify S-100.Recommended pairs for sandwich ELISA: Capture Detect 56203 56204 56206 56204
Immunogen Purified S-100 protein from human brain.
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions These antibodies are stable for at least one (1) year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.4, 0.09% NaN₃
Purity protein affinty purification
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2a - Isotype Control

target relevance
Protein names Protein S100-A1 (S-100 protein alpha chain) (S-100 protein subunit alpha) (S100 calcium-binding protein A1)
Gene names S100A1,S100A1 S100A
Protein family S-100 family
Mass 10546Da
Function FUNCTION: Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446). {ECO:0000269\|PubMed:11717446, ECO:0000269\|PubMed:12804600, ECO:0000269\|PubMed:22399290, ECO:0000269\|PubMed:23351007}.
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:15171681}. Sarcoplasmic reticulum {ECO:0000269\|PubMed:12804600}. Mitochondrion {ECO:0000250\|UniProtKB:P56565}.
Tissues TISSUE SPECIFICITY: Highly prevalent in heart (PubMed:12804600, PubMed:1384693). Also found in lesser quantities in skeletal muscle and brain (PubMed:1384693). {ECO:0000269\|PubMed:12804600, ECO:0000269\|PubMed:1384693}.
Structure SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (PubMed:21296671). Also forms heterodimers with S100P (PubMed:15171681). Interacts with AGER (By similarity). Interacts with CAPZA1 (By similarity). Interacts with FKBP4 (PubMed:20188096). Interacts with RYR1 and RYR2 (PubMed:18650434). Interacts with CACYBP in a calcium-dependent manner (PubMed:12042313). Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (PubMed:22399290). Interacts with ATP2A2 and PLN in a Ca(2+)-dependent manner (PubMed:12804600). Interacts with mitochondrial F1-ATPase subunits ATP5F1A and ATP5F1B; these interactions increase F1-ATPase activity (By similarity). {ECO:0000250\|UniProtKB:P35467, ECO:0000250\|UniProtKB:P56565, ECO:0000269\|PubMed:12042313, ECO:0000269\|PubMed:15171681, ECO:0000269\|PubMed:18650434, ECO:0000269\|PubMed:20188096, ECO:0000269\|PubMed:21296671, ECO:0000269\|PubMed:22399290}.
Post-translational modification PTM: Glutathionylated; glutathionylation increases affinity to calcium about 10-fold. {ECO:0000269\|PubMed:15885104}.
Target Relevance information above includes information from UniProt accession : P23297
The UniProt Consortium

Data

benchmark-antibodies_anti-s100alpha-beta_antibody_5838_1.jpg

20% PAGE w/ 2mM EGTA, 1 µg Antigen.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.