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mouse anti-S-100 monoclonal antibody (3B10) 1766

$338.00

Antibody summary

  • Mouse monoclonal to S-100
  • Suitable for: WB,ELISA,AP
  • Isotype: IgG2a
  • 100 µg
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SKU: 1766parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG2a

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

S100α/α

available sizes

100 µg

antibody
Tested applications
WB,ELISA
Recommended dilutions
These antibodies may be used in ELISA and Western blot (human brain): 56203, 56204, and 56206 may be used to affinity-purify S-100.Recommended pairs for sandwich ELISA: Capture Detect 56203 56204 56206 56204
Immunogen
Purified S-100 protein from human brain.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
These antibodies are stable for at least one (1) year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4, 0.09% NaN3
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG2a
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG2a - Isotype Control
target relevance
Protein names
Protein S100-A1 (S-100 protein alpha chain) (S-100 protein subunit alpha) (S100 calcium-binding protein A1)
Gene names
S100A1,S100A1 S100A
Protein family
S-100 family
Mass
10546Da
Function
FUNCTION: Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446). {ECO:0000269|PubMed:11717446, ECO:0000269|PubMed:12804600, ECO:0000269|PubMed:22399290, ECO:0000269|PubMed:23351007}.
Subellular location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15171681}. Sarcoplasmic reticulum {ECO:0000269|PubMed:12804600}. Mitochondrion {ECO:0000250|UniProtKB:P56565}.
Tissues
TISSUE SPECIFICITY: Highly prevalent in heart (PubMed:12804600, PubMed:1384693). Also found in lesser quantities in skeletal muscle and brain (PubMed:1384693). {ECO:0000269|PubMed:12804600, ECO:0000269|PubMed:1384693}.
Structure
SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (PubMed:21296671). Also forms heterodimers with S100P (PubMed:15171681). Interacts with AGER (By similarity). Interacts with CAPZA1 (By similarity). Interacts with FKBP4 (PubMed:20188096). Interacts with RYR1 and RYR2 (PubMed:18650434). Interacts with CACYBP in a calcium-dependent manner (PubMed:12042313). Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (PubMed:22399290). Interacts with ATP2A2 and PLN in a Ca(2+)-dependent manner (PubMed:12804600). Interacts with mitochondrial F1-ATPase subunits ATP5F1A and ATP5F1B; these interactions increase F1-ATPase activity (By similarity). {ECO:0000250|UniProtKB:P35467, ECO:0000250|UniProtKB:P56565, ECO:0000269|PubMed:12042313, ECO:0000269|PubMed:15171681, ECO:0000269|PubMed:18650434, ECO:0000269|PubMed:20188096, ECO:0000269|PubMed:21296671, ECO:0000269|PubMed:22399290}.
Post-translational modification
PTM: Glutathionylated; glutathionylation increases affinity to calcium about 10-fold. {ECO:0000269|PubMed:15885104}.
Target Relevance information above includes information from UniProt accession : P23297
The UniProt Consortium

Data

benchmark-antibodies_anti-s100alpha-alpha_antibody_1766_1.jpg
20% PAGE w/ 2mM EGTA, 1 µg Antigen.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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