| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2b |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Replication Factor C (p37) |
| available sizes | 100 µg |
mouse anti-Replication Factor C p37 monoclonal antibody (1320) 5771
$503.00
Antibody summary
- Mouse monoclonal to Replication Factor C p37
- Suitable for: WB,ICC/IF
- Isotype: IgG2b
- 100 µg
mouse anti-Replication Factor C p37 monoclonal antibody (1320) 5771
| antibody |
|---|
| Tested applications WB,ICC/IF |
| Recommended dilutions Immunoblotting: use at 1:2,000 dilution. A band of 37 kD is detected. Positive controls: HeLa cell lysate. |
| Immunogen Recombinant protein corresponding to the p37 subunit of human Replication Factor C expressed in E. coli. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity protein affinity purification |
| Clonality monoclonal |
| Isotype IgG2b |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2b - Isotype Control |
| target relevance |
|---|
| Protein names Replication factor C subunit 2 (Activator 1 40 kDa subunit) (A1 40 kDa subunit) (Activator 1 subunit 2) (Replication factor C 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40) |
| Gene names RFC2,RFC2 |
| Protein family Activator 1 small subunits family |
| Mass 39157Da |
| Function FUNCTION: Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA (PubMed:9488738). This subunit binds ATP (By similarity). {ECO:0000250, ECO:0000269|PubMed:9488738}. |
| Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. |
| Structure SUBUNIT: Subunit of the RFC complex, an heteropentameric complex consisting of a large subunit RFC1 and four small subunits RFC2, RFC3, RFC4 and RFC5; the RFC complex interacts with PCNA (PubMed:8999859, PubMed:9488738). Forms an heterotetrameric complex with RFC3, RFC4 and RFC5; this complex has ATPase activity but is not stimulated by PCNA (PubMed:9488738). The heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 interacts with RAD17 (PubMed:11572977). RFC2 also interacts with PRKAR1A; the complex may be involved in cell survival (PubMed:15655353). Interacts with DDX11 (PubMed:18499658). {ECO:0000269|PubMed:11572977, ECO:0000269|PubMed:15655353, ECO:0000269|PubMed:18499658}. |
| Involvement in disease DISEASE: Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region (PubMed:11003705). {ECO:0000269|PubMed:11003705}. |
| Target Relevance information above includes information from UniProt accession: P35250 |
| The UniProt Consortium |
Data
 |
| Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p37 antibody [1320] (5771) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
 |
| Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320](5771) antibody at 1:200 dilution. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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