| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Rad50 |
| available sizes | 100 µg |
mouse anti-Rad50 monoclonal antibody (2C6) 6741
$503.00
Antibody summary
- Mouse monoclonal to Rad50
- Suitable for: WB,ICC/IF,IHC-P,IP,IHC,in vitro
- Isotype: IgG1
- 100 µg
mouse anti-Rad50 monoclonal antibody (2C6) 6741
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF |
| Recommended dilutions Immunoblotting, Immunoprecipitation: use at 0.1-2 ug/mL. Positive control: Raji cells. |
| Immunogen GST fusion protein corresponding to the complete coding region (aa 1-425) of Rad50 expressed in E. coli. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. |
| Storage buffer PBS, pH 7.4 |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names DNA repair protein RAD50 (hRAD50) (EC 3.6.-.-) |
| Gene names RAD50,RAD50 |
| Protein family SMC family, RAD50 subfamily |
| Mass 153892Da |
| Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). |
| Subellular location Nucleus. Chromosome, telomere. Chromosome. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. |
| Tissues Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. |
| Structure Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN (PubMed:8756642, PubMed:9590181, PubMed:9705271, PubMed:10839544, PubMed:26215093). As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites (PubMed:26215093). Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN (PubMed:10783165). Found in a complex with TERF2 (PubMed:10888888). Interacts with RINT1 (PubMed:11096100). Interacts with BRCA1 via its N-terminal domain (PubMed:10426999). Interacts with DCLRE1C/Artemis (PubMed:15456891, PubMed:15723659). Interacts with MRNIP (PubMed:27568553). Interacts with CYREN (via XLF motif) (By similarity).; (Microbial infection) Interacts with herpes simplex virus 1 protein UL12 (PubMed:20943970). |
| Involvement in disease DISEASE: Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]: A disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: Q92878 |
| The UniProt Consortium |
Data
 |
| Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad50 protein stained by Rad50 antibody [13B3] (6741) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um. |
 |
| Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (6741) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 |
| Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (6741) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 |
| Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (6741) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 |
| Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (6741) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 |
| HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (6741) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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